导师风采
刘允军
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个人信息

Personal Information

  • 副研究员
  • 导师类别:硕士生导师
  • 性别: 男
  • 学历:博士研究生
  • 学位:博士

联系方式

Contact Information

  • 所属院系:作物科学所
  • 所属专业: 作物遗传育种
  • 邮箱 : liuyunjun@caas.cn
  • 工作电话 : 010-82105174

个人简介

Personal Profile

中国农业科学院作物科学研究所 副研究员。 2004在中国农业大学获得遗传学专业博士学位,2005-2007年在瑞典Lund 大学从事博士后工作,2007年进入中国农业科学院作物科学研究所工作至今。主要研究方向为玉米转基因与基因编辑、玉米籽粒发育分子机制解析。主持国家自然科学基金、生物育种课题、973任务等课题。以第一或通讯作者在Nucleic Acids Research, Plant Physiology, Journal Experimental Botany, Plant CellPhysiology等主流SCI期刊发表论文40余篇,获得第一发明人授权专利8项。


  • 研究方向Research Directions
玉米转基因与基因编辑,玉米籽粒发育分子机制解析
2. 机电结构优化与控制 研究内容:在对机电结构进行分析和优化的基础上,运用控制理论进行结构参数的调整,使结构性能满足设计要求。1. 仿生结构材料拓扑优化设计, 仿生机械设计 研究内容:以仿生结构为研究对象,运用连续体结构拓扑优化设计理论和方法,对多相仿生结构(机构)材料进行2. 机电结构优化与控制 研究内容:在对机电结构进行分析和优化的基础上,运用控制理论进行结构参数的调整,使结构性能满足设计要求。1. 仿生结构材料拓扑优化设计, 仿生机械设计 研究内容:以仿生结构为研究对象,运用连续体结构拓扑优化设计理论和方法,对多相仿生结构(机构)材料进行整体布局设计。 整体布局设计。
科研项目

1. 国家自然科学基金“ZmAlba6提高农杆菌遗传转化效率的分子机理解析”,起止时间2024.1-2027.12,经费50万元,主持人。

2. 生物育种课题“玉米高产耐密植等优异种质创制”,起止时间2022.12-2025.12,经费150万元,主持人。

3. 横向课题“玉米转基因技术开发研究”,起止时间2022.2-2026.1,经费300万元,主持人

4. 国家自然科学基金“SMK11表观遗传调控玉米籽粒发育的分子机制”,起止时间2019.1-2022.12,经费60万元,主持人。

5. 转基因重大专项任务“玉米规模化转基因技术体系构建”,起止时间2011.1-2020.12,经费615万元,主持人。

6. 中国农科院所级统筹科研业务费“基于新型不育系的玉米杂交育种技术的建立”(S2018QY07),起止时间2018.1-2020.12,经费90万元,主持人。

7. 科技部973任务“玉米品质性状的全基因组遗传解析”,起止时间2014.1-2018.12,经费67万元,主持人。

8. 教育部留学人员基金“玉米锌指蛋白基因ZmSAP13的功能及作用机制研究”,起止时间2010.1-2010.10, 经费4万元,主持人。

9. 转基因重大专项子课题“抗病虫、抗除草剂转基因玉米新品种培育”,起止时间2011.1-2020.12,经费1000万元,参加,主要执行人。


研究成果

1. Chen  QQ, Guo YM, Zhang J, Zheng NN, Wang J, Liu Y, Lu JW, Zhen SH, Du XM, Li L, Fu JJ, Wang GY, Gu RL*, Wang JH*, Liu YJ* (2023) RNA polymerase common subunit ZmRPABC5b is transcriptionally activated by Opaque2 and essential for endosperm development in maize. Nucleic Acids Research, 51:7832-7850.

2. Ren ZJ, Fan KJ, Zhen SH, Zhang J, Liu Y, Fu JJ, Qi QL, Wei QH, Du Y, Tatar W, Zhang XF, Wang GY, Rasmusson AG, Wang JH*, Liu YJ* (2023) Tetratricopeptide-containing SMALL KERNEL 11 is essential for the assembly of cytochrome c oxidase in maize mitochondria. Plant Physiology, 192:170-187.

3. Chen RR, Wei QH, Liu Y, Li JK, Du XM, Chen Y, Wang JH*, Liu YJ* (2023) The pentatricopeptide repeat protein EMP601 functions in maize seed development by affecting RNA editing of mitochondrial transcript ccmC. Crop Journal, 11:1368-1379.

4. Fan KJ, Fu QH, Wei QH, Jia SN, Zhao AQ, Wang TT, Cao J, Liu Y, Ren ZJ*, Liu YJ* (2022) ZmnMAT1, a nuclear-encoded type I maturase, is required for the splicing of mitochondrial Nad1 intron 1 and Nad4 intron 2 . Frontiers in Plant Science, 13:1033869.

5. Fan KJ, Ren ZJ, Zhang XF, Liu Y, Fu JJ, Qi CL, Tatar W, Rasmusson AG*, Wang GY*, Liu YJ* (2021) The pentatricopeptide repeat protein EMP603 is required for the splicing of mitochondrial Nad1 intron 2 and seed development in maize. Journal of Experimental Botany, 72: 6933–6948.

6. Xu QL, Yang L, Kang D, Ren ZJ, Liu YJ*  (2021) Maize MS2 encodes an ATP-binding cassette transporter that is essential for anther development. The Crop Journal,9:1301–1308.

7. Zhang J, Zhang X, Chen R, Yang L, Fan K, LiuY, Wang G, Ren Z*, Liu YJ* (2020) Generationof transgene-free semidwarf maize plants by gene editing of gibberellin-oxidase20-3using CRISPR/Cas9. Frontiers in Plant Science 11: 1048.

8. Du XM, FangT, Liu Y, Wang M, Zang MS, Huang LY, Zhen SH, Zhang J, Shi ZC, Wang GY, Fu JJ*, Liu YJ* (2020) Global profiling of N6-methyladenosine methylation in maize callusinduction. Plant Genome e20018

9. Du XM, FangT, Liu Y, Huang LY, Wang XL, Zhang J, Cui YB, Zang MS, Wang GY, Fu JJ*, Liu YJ* (2020) Global profiling of alternative splicing in callusinduction of immature maize embryo. InVitro Cell Dev Bioy –Plant,56:159–168

10. Ren Z, Fan K, Fang T, Zhang J,Yang L, Wang J, Wang G*, Liu YJ* (2019) Maize Empty pericarp602 encodes a P-type PPR protein thatis essential for seed development. Plant Cell Physiology 60(8): 1734-1746.

11. Du X, Fang T, Liu Y, Huang L, Zang M, Wang G,Liu YJ*, Fu J* (2019) Transcriptome profiling predicts new genes to promote maize callus formationand transformation. Frontiers in Plant Science 10: 1633.

12. Chen R, Xu Q, Liu Y, Zhang J, Ren D, Wang G, LiuYJ* (2018) Generation of transgene-free maize male sterile lines usingthe CRISPR/Cas9 system. Frontiers in Plant Science 9: 1180.

13. Liu Y, Zhang Z, Fu J, Wang G, Wang J, LiuYJ * (2017) Transcriptome analysis of maize immature embryos revealsthe roles of cysteine in improving Agrobacterium infection efficiency. Frontiersin Plant Science  8: 1778.

14. Kang D, Wang CY, Xu QL, Wang GY, LiuYJ* (2017) Characterization of maize malesterile2 mutant by phenotypic and RNA sequencing analyses. Plant Breeding.  136:319-330.

15. Cao Z, Zhang W, Ning X, Wang B*, Liu YJ*, Li QX* (2017) Development of monoclonal antibodies recognizing linear epitope:Illustration by three Bacillusthuringiensis crystal proteins of genetically modified cotton, maize, and tobacco.Journal of Agricultural and Food Chemistry 65(46): 10115-10122.

16. LiuYJ, Zhang YW, Liu Y, Lu W, Wang GY (2015)Metabolic effects of glyphosate on transgenic maize expressing a G2-EPSPS gene from Pseudomonasfluorescens. Journal of Plant BiochemistryBiotechnology,24:233-241.

17. Liu YJ#, Cao GY#, Chen RR, ZhangSX, Ren Y, Lu W, Wang JH, Wang GY (2015) Transgenic tobacco simultaneously overexpressing glyphosateacetyltransferase and 5-enolpyruv-ylshikimate-3-phosphate synthase are moreresistant to glyphosate than those containing one gene. Transgenic Research. 24:753-763.

18. Ren ZJ, Cao GY, Zhang YW, Liu Y, LiuYJ* (2015) Overexpression of a modified AM79aroA gene in transgenic maize confers high tolerance to glyphosate. Journal of Integrative Agriculture, 14:414-422

19. Ren ZJ, Liu Y, Kang D, Fan KJ, Wang CY, Wang GY, Liu YJ (2015) Two alternative splicing variants of maize HKT1;1confer salt tolerance in transgenic tobacco plants Plant Cell Tissue Organ Culture, 123:569-578.

20. Yuan Y, Qi LJ, Yang J, Wu C, LiuYJ*, Huang LQ* (2015) A Scutellaria baicalensis R2R3-MYB gene, SbMYB8,regulates flavonoid biosynthesis and improves drought stress tolerance intransgenic tobacco. Plant Cell TissueOrgan Culture , 120:961-972.

21. Zhang YW, Liu Y, Zhang J, Wang GY, WangJH, Liu YJ* (2015) Assessmentof transgene copy number and zygosity of transgenic maize overexpressing Cry1Iegene with SYBR® Green qRT-PCR. In VitroCellular and Developmental Biology–Plant, 51:125 -134

22.Zhang YW#,Liu YJ#, Ren Y, Liu Y,Liang GM, Song FP, Bai SX, Wang JH, Wang GY (2013) Overexpression of a novel Cry1Ie gene confers resistance toCry1Ac-resistant cotton bollworm in transgenic lines of maize. Plant Cell Tissue Organ Culture, 115:151-158.

23.Zhang SX,Lian Y, Liu Y, Wang XQ,LiuYJ*,Wang GY* (2013) Characterization of a maizewip1 promoter in transgenic plants. InternationalJournal of Molecular Sciences, 14:23872-23892.

24.Yuan Y, Wu C,Liu YJ*, Yang J, Huang LQ* (2013) The Scutellariabaicalensis R2R3 MYB transcription factors modulates flavonoid biosynthesisby regulating GA metabolism in transgenic tobacco plants. PLoS ONE,8:e77275.

25.Liu YJ, Yuan Y, Liu YY, Liu Y, Fu JJ,Zheng J, Wang GY (2012) Gene families of maizeglutathione–ascorbate redox cycle respond differently to abiotic stresses Journal of Plant Physiology, 169:183-192.

26. Cao GY#, Liu YJ#, Zhang SX, Yang XW, Chen RR,Zhang YW, Lu W, Wang JH, Lin M, Wang GY (2012) A novel 5-nolpyruvylshikimate -3-phosphate synthase shows high glyphosatetolerance in Escherichia coli and tobacco plants.  PLoS ONE,7:e38718.

27. Yuan Y#, Liu YJ#, Wu C, Chen S, Wang ZY, Yang ZC, Qin SS,Huang LQ (2012) Water deficit affected flavonoid accumulation byregulating hormone metabolism in Scutellaria baicalensis Georgi roots. PLoS ONE,7:e42946.

28. Liu YJ, Nunes-Nesi A,Wallstrom SV, Lager I, Michalecka AM, Norberg FE, Widell S, Fredlund KM, FernieAR, Rasmusson AG (2009) A redox-mediated modulation of stem boltingin transgenic Nicotiana sylvestris differentially expressing the external mitochondrialNADPH dehydrogenase. Plant Physiology,150:1248-1259.

授权专利

1. 刘允军,刘艳,王国英,任珍静,陈岩,施建琴. 一种转基因玉米VB15外源插入片段的旁侧序列及其应用 (专利号 ZL202311302047.9)

2. 刘允军,王国英,刘艳. 一种抗虫转基因玉米AM63插入位点的外源插入片段的旁侧序列及其应用 (专利号 ZL202110401503.X)

3. 刘允军,刘艳,王国英. 玉米BBM1基因在提高植物遗传转化效率中的应用 (专利号 201910411582.6)

4. 刘允军,王国英,刘艳.一种农杆菌侵染玉米幼胚的方法(专利号201310022133.4)

5.刘允军,王国英,刘艳.一种通过弱光培养体系快速获得转基因植物的方法 (专利号201710090814.2)

6.刘允军,王国英,张煜文,刘艳. 融合杀虫蛋白Cry1Am、其编码基因及应用(专利号201510175515.X)

7.刘允军,张煜文,王国英,何康来.一种杀虫蛋白及其编码基因与应用( 专利号201410591794.3)

8.刘允军,任珍静,王国英. 一种玉米耐盐基因及其应用 (专利号201410838479.6)


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