国家自然科学基金委员会，面上项目，32171453，基于Wus2/Bbm和Ac/Ds的可控型剔除标记基因的植物高效转化体系研究，2022-01至2025-12，58万元，在研，主持

国家自然科学基金委员会，青年基金项目，31500301，基于双向启动子和2A连接肽的玉米多基因表达策略研究，2016-01至2019-12，25万元，已结题，主持

主要论著

1. Sun M, Li S, Yang W, Zhao B, Wang Y*, Liu X*. Commercial genetically modified corn and soybean are poised following pilot planting in China. Molecular Plant. 2024 Apr 1;17(4):519-521.

2. Ma X†, Yue Q†, Miao L†, Li S†, Tian J, Si W, Zhang L, Yang W, Zhou X, Zhang J, Chen R*, Xu Y*, Liu X*. A novel nucleic acid linker for multi-gene expression enhances plant and animal synthetic biology. The Plant Journal. 2024 Jun;118(6):1864-1871.

3. Chen C†, Liu X†, Li S, Liu C, Zhang Y, Luo L, Miao l, Yang W, Xiao Z, Zhong Y, Li J, Chen R,* and Chen S*. Co-expression of transcription factors ZmC1 and ZmR2 establishes an efficient andand accurate haploid embryo identification system in maize. Plant Journal (2022), doi: 10.1111/tpj.15888

4. Liu X, Ma X, Wang H, Li S, Yang W, Ramdhan DN, Luo L, Zhou X, Tang C, Fan Y, Zhao Q, Zhang J*, and Chen R*. Metabolic engineering of astaxanthin-rich maize and its use in the production of biofortified eggs. Plant Biotechnology Journal (2021), Sep;19(9):1812-1823.

5. Liu X, Yang W, Mu B, Li S, Li Y, Zhou X, Zhang C, Fan Y, Chen R*. Engineering of 'Purple Embryo Maize' with a multigene expression system derived from a bidirectional promoter and self-cleaving 2A peptides. Plant Biotechnology Journal (2018), Jun;16(6): 1107–1109.

6. Liu X†, Li S†, Yang W, Mu B, Jiao Y, Zhou X, Zhang C, Fan Y, Chen R*. Synthesis of seed-specific bidirectional promoters for metabolic engineering of anthocyanin-rich maize. Plant Cell Physiol(2018),  Oct 1;59(10):1942-1955.

7. Liu X, Yang W, Li Y, Li S, Zhou X, Zhao Q, Fan Y, Lin1, M* and Chen R*. The intergenic region of the maize defensin-like protein genes Def1 and Def2 functions as an embryo-specific asymmetric bidirectional promoter. Journal of Experimental Botany(2016), 67 (14):4403-4413.

8. Liu X, Tian J, Zhou X, Chen R, Wang L, Zhang C, Zhao J, Fan Y. Identification and characterization of promoters specifically and strongly expressed in maize embryos. Plant Biotechnology Journal(2014) Dec;12(9):1286-96. 

9. Liu X, Zhou X, Li Y, Tian J, Zhang Q, Li S, Wang L, Zhao J, Chen R*, Fan Y. Identification and functional characterization of bidirectional gene pairs and their intergenic regions in maize. BMC Genomics(2014) May 5;15:338. 

10. Li S†, Liu X†, Xiaojin Zhou, Ye Li, Wenzhu Yang and Rumei Chen*. Improving Zinc and Iron Accumulation in Maize Grains Using the Zinc and Iron Transporter ZmZIP5. Plant Cell Physiol(2019), Sep 1;60(9):2077-2085.

11. Ma S, Yang W, Liu X, Li S, Li Y, Zhu J, Zhang C, Lu X, Zhou X, Chen R*. Pentatricopeptide repeat protein CNS1 regulates maize mitochondrial complex III assembly and seed development. Plant Physiology(2022), Jun 1;189(2):611-627.

12. Li S, Song Z, Liu X, Zhou X, Yang W, Chen J, Chen R*. Mediation of Zinc and Iron Accumulation in Maize by ZmIRT2, A Novel Iron-Regulated Transporter. Plant Cell Physiol(2022), Apr 19;63(4):521-534.

13. Li Y, Liu X, Li J, Li S, Chen G, Zhou X, Yang W, Chen R*. Isolation of a maize ZmCI-1B promoter and characterization of its activity in transgenic maize and tobacco. Plant Cell Reports(2015), Aug;34(8):1443-57. 

14. Zhou X, Li S, Zhao Q, Liu X, Zhang S, Sun C, Fan Y, Zhang C, Chen R*. Genome-wide identification, classification and expression profiling of nicotianamine synthase (NAS) gene family in maize. BMC Genomics. 2013 Apr 10;14:238.

授权发明专利

1. 柳小庆，苗丽青，田健，陈茹梅，李素贞，周晓今，杨文竹. gat3基因及其突变体在培育抗草甘膦作物中的应用. ZL202210423669.6

2. 柳小庆、陈茹梅、杨文竹、范云六、周晓今、李业、李素贞。种子特异性双向启动子及其应用. ZL201810097695.8

3. 柳小庆、陈茹梅、李素贞、杨文竹、周晓今. 一种联合表达载体及其在玉米籽粒表达虾青素中的应用. ZL202011093167.9 

4. 陈茹梅、陈绍江、李建生、柳小庆、陈琛、马帅、刘晨旭. 一种快速显色孤雌生殖诱导系及其在鉴别玉米单倍体中的应用. ZL20211088418308

5. 王磊、柳小庆、范云六、 陈茹梅、赵军、田健、张春义. 玉米组织特异性启动子及其应用.ZL201510038836.5.

6. 陈茹梅、柳小庆、范云六、李业、赵军、田健、王磊、张春义. 从玉米中分离的双向启动子及其应用. ZL201410683241.0.

7. 陈茹梅、柳小庆、范云 六、李业、周晓今、赵军、田健、王磊. 玉米组织特异性启动子及其应用. ZL201410683244.4.

8. 陈茹梅、柳小庆、范云六、李业、周晓今、赵军、田健、王磊. 从玉米中分离的组织特异性启动子及其应用.ZL201410682994. X.  

9. 王磊、范云六、柳小庆、陈茹梅、赵军、周晓今、田健、 李业. 玉 米 组 织 特 异 性 启 动 子 及 其 在 提 高 作 物 种 子 品 质 中 的 应 用.ZL201410683469.X.

10.  田健、范云六、柳小庆、 赵军、陈茹梅、王磊、张春义、伍宁丰. 玉米组织特异性启动子及其应用.ZL201410429204.7.