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单同领,博士,研究员,猪传染病研究室副主任,2019年入选上海市领军人才后备队培养计划, 2020年荣获中国农业科学院脱贫攻坚与乡村振兴先进个人称号,2020年入选中国农业科学院所级青年英才培育计划,猪病毒性繁殖障碍综合征创新团队一级科研骨干。
主要从事新发和再现病毒的基础和应用研究,瞄准生物安全和畜牧业疫病防控,在国内率先建立病毒宏基因组学快速诊断新发疫病平台,完成200多种野生动物和家养动物体内病毒群落,把前沿生物技术和疫病防控关键技术相结合,以冠状病毒和微小RNA病毒为模型,解析病毒和宿主相互博弈的分子机制,开展创新疫苗和诊断试剂研究。
先后主持国家重点研发计划“生物安全关键技术研发专项”课题、“新发与再现畜禽重大疫病的致病与免疫机制研究”子课题和国家自然科学基金等项目,累计经费1000余万元,发表SCI论文80余篇,其中以第一作者或通讯作者,在Autophagy、Journal of Virology、MicrobiologySpectrum和Emerging infectionDisease等杂志发表SCI论文近40篇,单篇被引用200余次;授权专利3项;获得兽用生物制品临床试验批件1个;成果转化到账400万元。
工作经历:
2011.8-2013.12 中国农业科学院上海兽医研究所 助理研究员
2014.1-2018.12 中国农业科学院上海兽医研究所 副研究员
2019.1-至今 中国农业科学院上海兽医研究所 研究员
2020.8-2020.10 西藏林芝市农业农村局
2021.1-至今 中国农业科学院上海兽医研究所 副主任
学习经历:
2001.9-2005.7 河南农业大学牧医工程学院, 动物医学,学士学位
2005.9-2008.6 华中农业大学动医动科学院, 临床兽医学,硕士学位
2006.6-2008.1 中国农业科学院北京畜牧所
2008.9-2011.6 上海交通大学农业与生物学院,生物医学工程,博士学位
2010.5-2011.5 加州大学旧金山分校, 分子病毒学,访问学者
1. 国家自然科学基金面上项目,31872478,GSK3介导β-catenin和N蛋白调控猪流行性腹泻病毒复制的分子机制研究,2019/01-2022/12, 70.8万元,在研,主持
2. 科技部,国家重点研发计划课题,2017YFC1200201,重要疫源动物微生群落与未知新发病原的耐药致病性和适应性进化,2017/07-2020/06,590万元,结题,主持
3. 科技部,国家重点研发计划子课题,2016YFD0500103,猪冠状病毒性腹泻的免疫保护机制研究,2016/07-2020/12,140万,结题,主持子课题
4. 国家自然科学基金青年项目,31201915, 新发仔猪腹泻病因的筛选及鉴定,2013/01-2015/12,25万元,结题,主持
5. 上海市科技兴农重点攻关项目,沪农科攻字(2013)第3-6号,猪消化道病原口服疫苗研究,2013/10-2017/4,32万元,结题,主持子课题
6. 上海市科技兴农重点攻关项目,沪农科攻字(2013)第5-5号,上海地区仔猪腹泻病原生态学及防控技术研究,2013/11-2016/11,60万元,结题,主持子课题
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支持扩展名:.rar .zip .doc .docx .pdf .jpg .png .jpeg1. 国家自然科学基金面上项目,31872478,GSK3介导β-catenin和N蛋白调控猪流行性腹泻病毒复制的分子机制研究,2019/01-2022/12, 70.8万元,在研,主持
2. 科技部,国家重点研发计划课题,2017YFC1200201,重要疫源动物微生群落与未知新发病原的耐药致病性和适应性进化,2017/07-2020/06,590万元,结题,主持
3. 科技部,国家重点研发计划子课题,2016YFD0500103,猪冠状病毒性腹泻的免疫保护机制研究,2016/07-2020/12,140万,结题,主持子课题
4. 国家自然科学基金青年项目,31201915,新发仔猪腹泻病因的筛选及鉴定,2013/01-2015/12,25万元,结题,主持
5. 上海市科技兴农重点攻关项目,沪农科攻字(2013)第3-6号,猪消化道病原口服疫苗研究,2013/10-2017/4,32万元,结题,主持子课题
6. 上海市科技兴农重点攻关项目,沪农科攻字(2013)第5-5号,上海地区仔猪腹泻病原生态学及防控技术研究,2013/11-2016/11,60万元,结题,主持子课题
1. Ning Kong* , Tongling Shan* , HuaWang, Yajuan Jiao , Yewen Zuo , Liwei Li, WuTong, Lingxue Yu, Yifeng Jiang , YanjunZhou , Guoxin Li, Fei Gao, Hai Yu , HaoZheng , Guangzhi Tong#. BST2 Suppresses Porcine EpidemicDiarrhea Virus Replication by Targeting and Degrading Virus NucleocapsidProtein With Selective Autophagy. Autophagy. 2020;16(10):1737-1752. doi:10.1080/15548627.2019.1707487
2. Hua Wang*, Ning Kong*, Yajuan Jiao*, SujieDong*, Dage Sun, Xiaoyong Chen, Hao Zheng, Wu Tong, Hai Yu, Lingxue Yu, WenZhang, Guangzhi Tong#, Tongling Shan#.EGR1suppresses porcine epidemic diarrhea virus replication by regulating IRAV todegrade viral nucleocapsid protein. J Virol. 2021 Jul 21:JVI0064521. doi:10.1128/JVI.00645-21. Epub ahead of print. PMID: 34287043.
3. Yajuan Jiao*,Ning Kong*, Hua Wang*, Dage Sun*, Sujie Dong, Xiaoyong Chen, Hao Zheng, WuTong, Hai Yu, Lingxue Yu, Yaowei Huang, Huan Wang, Baokun Sui, Ling Zhao, YingLiao, Wen Zhang, Guangzhi Tong #, Tongling Shan#.PABPC4 broadly inhibitscoronavirus replication by degrading nucleocapsid protein through selectiveautophagy. Microbiology Spectrum. 2021, 6:e0090821. doi:10.1128/Spectrum.00908-21.
4. Shixing Yang* , Tongling Shan* , Yuqing Xiao*, HetengZhang , Xiaochun Wang , Quan Shen , YanWang , Yuxin Yao , Qi Liu , HaoWang #, Wen Zhang#. Digging metagenomic data of pangolinsrevealed SARS-CoV-2 related viruses and other significant viruses. J Med Virol. 2020Sep 17. doi: 10.1002/jmv.26524. Online ahead of print.
5. Chuanfeng Li*, Tongling Shan*, Zongyan Chen, Jiewen Zhou, Hang Li, Guangzhi Tong#,Guangqing Liu#.Isolation and complete genome analysis of a novel duckpicornavirus in China. Veterinary Microbiology.2021 Feb;253:108950. doi: 10.1016/j.vetmic.2020.108950
6. Xiaoyong Chen, Dage Sun, SujieDong, Huanjie Zhai, Ning Kong, Hao Zheng, WuTong, Guoxin Li, Tongling Shan#, GuangzhiTong#. Host Interferon-Stimulated Gene 20Inhibits Pseudorabies Virus Proliferation. VirolSin. 2021 Apr 8. doi: 10.1007/s12250-021-00380-0
7. Hua Wang*, Xiaoyong Chen*, NingKong , Yajuan Jiao, Dage Sun, Sujie Dong, WenzhenQin, Huanjie Zhai , Lingxue Yu, Hao Zheng, WuTong, Hai Yu, Guangzhi Tong #, Tongling Shan #.TRIM21 inhibits porcine epidemicdiarrhea virus proliferation by proteasomal degradation of the nucleocapsidprotein. Arch Virol. 2021 Apr26;1-9. doi: 10.1007/s00705-021-05080-4
8. Yuqing Xiao* , HaoWang* , Lei Feng , Jiamin Pan , ZeyuChen , Huiling Wang 1, Shixing Yang , QuanShen , Xiaochun Wang , TonglingShan #, WenZhang# . Fecal, oral, blood and skin viromeof laboratory rabbits. Arch Virol. 2020Dec;165(12):2847-2856. doi: 10.1007/s00705-020-04808-y
9. Xiaoyong Chen , NingKong, Jingjing Xu, Juan Wang, Mingliang Zhang, KeyueRuan, Liwei Li , Yujiao Zhang , HaoZheng , Wu Tong, Guoxin Li, Tongling Shan #, Guangzhi Tong #. Pseudorabiesvirus UL24 antagonizes OASL-mediated antiviral effect. VirusRes. 2021 Apr 2;295:198276. doi: 10.1016/j.virusres.2020.198276.
10. Ning Kong* , QiongMeng* , Yajuan Jiao* , Yongguang Wu, Yewen Zuo Hua Wang, Dage Sun , Sujie Dong , HuanjieZhai , Wu Tong , Hao Zheng , HaiYu , Guangzhi Tong , Yongjie Xu #, Tongling Shan#.Identification ofa novel B-cell epitope in the spike protein of porcine epidemic diarrhea virus.Virol J. 2020 Apr 3;17(1):46.
10. doi:10.1186/s12985-020-01305-1.
11. Muddassar Hameed*, AbdulWahaab*, Tongling Shan*, Xin Wang , Sawar Khan, DiDi , Liu Xiqian, Jun-Jie Zhang , Muhammad NaveedAnwar , Mohsin Nawaz , Beibei Li, KeLiu , Donghua Shao , Yafeng Qiu , Jianchao Wei#, Zhiyong Ma#. A MetagenomicAnalysis of Mosquito Virome Collected From Different Animal Farms atYunnan-Myanmar Border of China. Front Microbiol. 2021Feb 8;11:591478. doi: 10.3389/fmicb.2020.591478. eCollection 2020.
12. Wang H*, Ling Y*, Shan T*, Yang S, Xu H, Deng X, Delwart E, ZhangW#. Gut virome ofmammals and birds reveals high genetic diversity of thefamily Microviridae. Virus Evol. 2019, 8;5(1):vez013. SCI, IF=5.4
13. Wang H, Yang S, Shan T*, Wang X, Deng X, Delwart E, Zhang W*. A novel picornavirus in feces of a rainbow lorikeet(Trichoglossus moluccanus) shows a close relationship to members of the genusAvihepatovirus. Arch Virol. 2019;164(7):1911-1914.
14. Zhang Y, Shan TL#, Li F, Yu T, Chen X, Deng XT, Delwart E, Feng XP#. A novel phage from periodontal pockets associated withchronic periodontitis. Virus Genes. 2019; 55(3):381-393
15. Wang H, Zhang W, Yang S, Kong N, Yu H,Zheng H, Gao F, Tong W, Li L, Wang X, Deng X, Delwart E, Shan T#. Asian black bear (Ursus thibetanus) picornavirus relatedto seal aquamavirus A. Arch Virol. 2019Feb;164(2):653-656. doi: 10.1007/s00705-018-4101-6.
16. Wang H, Li S, Mahmood A, Yang S, Wang X,Shen Q, Shan T(通讯作者), Deng X, Li J,Hua X, Cui L, Delwart E, Zhang W. Plasma virome of cattle from forest region revealeddiverse small circular ssDNA viral genomes. Virol J. 2018.15;15(1):11.
17. Yan Wang,Wen Zhang, Zhijian Liu, Xingli Fu, Jiaqi Yuan,Jieji Zhao, Yuan Lin, Quan Shen, Xiaochun Wang,Xutao Deng, Eric Delwart, Tongling Shan(共同通讯作者).Full-length and defective enterovirus G genomes with distinct torovirusprotease insertions are highly prevalent on a Chinese pig farm. Archives ofVirology. 2018 Sep;163(9):2471-2476
18. Qi D*, Shan T*,Liu Z, Deng X, Zhang Z, Bi W, Owens JR, Feng F, Zheng L, Huang F, Delwart E,Hou R, Zhang W. A novelpolyomavirus from the nasal cavity of a giant panda (Ailuropoda melanoleuca).Virol J. 2017 Oct 27; 14(1):207.
19. Zhang Y, Li F, Chen X, Shan TL(通讯作者), Deng XT, DelwartE, Feng XP. Detection ofa new species of torque teno mini virus from the gingival epithelium ofpatients with periodontitis. Virus Genes. 2017 Sep 2. doi:10.1007/s11262-017-1505-4.
20. Zhang Y, Li F, Shan TL(通讯作者), DengX, Delwart E, Feng XP. A novelspecies of torque teno mini virus (TTMV) in gingival tissue from chronicperiodontitis patients. Sci Rep. 2016, 25; 6:26739;SCI,IF=5.3
21. Kong N, Wu Y, Meng Q, Wang Z, Zuo Y, PanX, Tong W, Zheng H, Li G, Yang S, Yu H, Zhou EM, Shan T(通讯作者), Tong G.. Suppression of Virulent PorcineEpidemic Diarrhea Virus Proliferation by the PI3K/Akt/GSK-3α/β Pathway.PLoS One. 2016,25;11(8):e0161508
22. Ning Kong , Yewen Zuo , Zhongze Wang , Hai Yu , En-Min Zhou , Tongling Shan*, GuangzhiTong*. Molecularcharacterization of new described kobuvirus in dogs with diarrhea in China. Springerplus. 2016 Nov 30;5(1):2047. doi: 10.1186/s40064-016-3738-4
23. Shan T, Li L, Simmonds P, Wang C, Moeser A,Delwart E. The fecal virome of pigs on a high-density farm. J Virol. 2011.; 85(22):11697-708. SCI, IF=5.189
24. Shan TL, Lan DL, Li LL, Wang CM, Cui L, Zhang W,Hua XG, Zhu CX, Zhao W, Delwart E. Genomic characterization and high prevalenceof bocaviruses in swine. PLoS One.2011, 6(4): e17292. SCI, IF=4.41
25. Shan T, Wang C, Cui L, Yu Y, Delwart E, Zhao W,Zhu C, Lan D, Dai X, Hua X. Picornavirus salivirus/klassevirus in children withdiarrhea, China. Emerg Infect Dis.2010, 6(8):1303-5. SCI, IF=6.859
26. Shan TL, Wang CM, Cui L,Delwart E, Yuan CL, Zhao W, Guo W, Dai XQ, Yu Y, Hua XG. Human parechovirusinfections in monkeys with diarrhea, China. Emerg Infect Dis. 2010, 16(7):1168-9. SCI, IF=6.859
27. Tongling Shan, Chunmei Wang, WuTong, Hao Zheng, Xiuguo Hua, Shen Yang, Yifei Guo, Wen Zhang, Guangzhi Tong. Complete Genome of a Novel PorcineAstrovirus. J Virol, 2012; Accept. SCI, IF=5.4
28. Shan TL, Zhang W, Guo W, Cui L, Yuan CL, Dai XQ,Shen Q, Yang ZB, Zhu JG, Hua XG. The first detection of human bocavirus 2infections in China. J Clin Virol. 2009, 46(2):196-7. SCI, IF=4.023
29. Shan TL, Guo W, Cui L, Shang XG, Dai XQ, Yuan CL,Yu Y, Zhang W, Zhu JG, Shen Q, Yang ZB, Hua XG. The first detection of humanparechovirus infections in China. J Clin Virol. 2009, 5(4):371-2. SCI, IF=4.023
30. Shan TL, Dai XQ, Guo W, Zhang W, Cui L, Yuan CL,Shang XG, Shen Q, Hua XG. Human astrovirus infection in children withgastroenteritis in Shanghai. J Clin Virol. 2009, 44(3):248-9. SCI, IF=4.023
31. Shan T, Cui L, Dai XQ,Guo W, Shang XG, Yu Y, Zhang W, Kang YJ, Shen Q, Yang ZB, Zhu JG, Hua XG.Sequence analysis of an isolate of minute virus of canines in China reveals theclosed association with bocavirus. Mol Biol Rep. 2010, 37(6):2817-20. SCI, IF=1.875
32. Shan TL, Tang ZL, Guo DZ, Yang SL, Mu YL, Ma YH,Guan WJ, Li K. Partial molecular cloning, characterization, and analysis of thesubcellular localization and expression patterns of the porcine OTUB1 gene. MolBiol Rep. 2009, 36(6):1573-7. SCI,IF=1.875
33. Shan TL, Li K, Tang ZL, Yang SL, Ma YH, Guan WJ,Guo DZ. Full-length coding sequences, polymorphism and chromosomallocalizations of the porcine EDG4 and EDG7 genes. Mol Biol Rep. 2009,36(4):751-6. SCI, IF=1.875
34. Zhu CX*, Shan TL*, Cui L, Luo XN,Liu ZJ, Tang SD, Liu ZW, Yuan CL, Lan DL, Zhao W, Hua XG. Molecular detectionand sequence analysis of feline Torque teno virus (TTV) in China. Virus Res.2011, 56:13-6. SCI, IF=2.905
35. Yu Y*, Shan T*, Zhu S, Zhu C,Hua X, Wang C. Prokaryoteexpression of HPeV-1 VP1 protein, production of VP1 polyclonal antibody and thedevelopment of an ELISA. J Virol Methods. 2012, 182(1-2):56-61. SCI, IF=2.139.
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