个人信息
Personal Information
联系方式
Contact Information
个人简介
Personal Profile
和玉兵，男，博士，博士生导师，中国农业科学院作物精准育种技术团队执行首席，入选中国农业科学院农科英才“优秀青年英才”。2010年本科毕业于华中农业大学生命科学技术学院国家理科基地班-生物技术，2017年博士毕业于华中农业大学生命科学技术学院。围绕作物转基因与基因编辑瓶颈，聚焦简化作物基因编辑材料创制全流程，取得主要业绩①率先开发了转基因自杀基因编辑技术（Transgene Killer CRISPR，TKC）等多种清除转基因的基因编辑方案。利用TKC技术收获T0种子即为无转基因的基因编辑种子，无需进行后代转基因鉴定，省时省力。进一步，TKC实现一代创制无转基因残留多基因编辑材料，升级后的RUBY-TKC实现转基因消除率达100%，为作物基因编辑育种提供了安全有效的技术支撑。并针对纯合突变致死或不育等“必需基因”纯合敲除突变体无法传代问题，开发错配靶标鸡尾酒策略（TKC-MC），为系统性突变水稻所有基因提供有效技术基础。TKC技术目前被国内外百余家实验室用于创制遗传稳定无转基因的基因编辑材料。②率先开发了可见光下裸眼可视新型报告系统（RUBY），相关研究发表ESI高被引论文（单篇被引超500次），该系统已成功助力水稻、小麦、玉米、大豆、高粱等70余种植物简化转基因、基因编辑。基于RUBY，开发了大豆免组培快速转化方法，突破了大豆基因型限制，不需无菌组培，简化操作流程，将转基因培育周期压缩至40-80天，为大豆转基因及基因编辑育种提供了有效的技术支撑。RUBY报告系统已被国内外千余家实验室用于相关研究，2024年Nature“未来技术”专栏撰文《Twenty years of Addgene》报道其为Addgene最热门植物类载体之一 (https://www.nature.com/articles/d41586-024-03152-4)。③通过对微型基因编辑工具IscB蛋白（仅含496个氨基酸，更易于递送至植物细胞）及其引导RNA进行了多重工程化优化与组合，解决天然IscB系统在植物中的编辑效率较低等问题，构建IscB等紧凑型植物基因编辑系统，畅通编辑工具递送。累计在Molecular Plant、Plant Biotechnology Journal、Plant Communications等刊物发表基因编辑相关论文三十余篇。
国家自然科学基金，主持，在研；
中国农业科学院青年创新专项，主持，在研；
中国农业科学院南繁专项，主持，在研。
* Corresponding author, # Co-first author, † Senior author
1. Xu M, Yan L, Zhu M, Zhan Z, Chen H, Wang D, Zheng Z, Zhang Y, Xiong L, He Y*. TKC-MC: An Effective Strategy for Generating Heritable Heterozygous Mutations in Essential Genes in Rice. Plant Biotechnology Journal, 2026, 24(4): 2092-2104.下载
2. Li Y#, Li C#, Yang J, Sun X, Li J, Yan L, Zhang C, Li S, He Y*, Xia L*. Engineering the hypercompact miniature IscB-ωRNA systems for efficient rice genome editing. Plant Communications, 2026, 7(1): 101562.下载
3. Li Y#, Lu K#, Feng S, Ning R, Li H, Zhao X, He Y*, Han Y*. ROTIS: A RUBY-assisted one-shot tissue-culture-free system for in-planta soybean transformation from germinated seedlings. Plant Physiology, 2025, 199(4).下载
4. Liu JL#, Yang T#, Fu YW, Zhan Z, Chen H, Cheng H, Zhou J, Wang YM, Tang LJ, Chen WQ, Wu MW, Zhao D, Liu CM, He Y*, Liu J*. Seeing Red, Selecting True: RUBY-Reported Seed Marker Streamlines CRISPR-Clean Rice Breeding. Rice (N Y), 2025, 18: 91.下载
5. Zhu M, Yan L, Zhan Z, Chen H, Wang D, Xu M, Zheng Z, Zhang Y, Yang N, Wu J, Zhan H, Tian Y, Xiong L, He Y*. Transgene-Killer-CRISPR version 2 (TKC2) eliminates occasional transgene escape by coupling with a RUBY reporter. Plant Biotechnology Journal, 2025, 23(10): 4621-4632.下载
6. Mu Q, Yu G, Zhou G, He Y*, Zhang J*. DRBP-EDP: classification of DNA-binding proteins and RNA-binding proteins using ESM-2 and dual-path neural network. NAR Genomics and Bioinformatics, 2025, 7(2): lqaf058.下载
7. Yang N#, Yan L#, Zheng Z, Zhang Y, Zhan H, Tian Y, Zhang T, Li R, Gong X, Xu M, Zhu M, Wu J, He Y*, Zhao Y. Editing gene families by CRISPR/Cas9: accelerating the isolation of multiple transgene-free null mutant combinations with much reduced labor-intensive analysis. Plant Biotechnology Journal, 2022, 20(2): 241-243.下载
8. He Y, Mudgett M, Zhao Y*. Advances in gene editing without residual transgenes in plants. Plant Physiology, 2022, 188(4): 1757-1768.下载
9. Liu JL, Chen MM, Chen WQ, Liu CM, He Y†, Song XF†*. A CASE toolkit for easy and efficient multiplex transgene-free gene-editing. Plant Physiology, 2022, 188(4): 1843-1847.下载
10. He Y, Yan L, Ge C, Yao XF, Han X, Wang R, Xiong L, Jiang L, Liu CM, Zhao Y*. PINOID Is Required for Formation of the Stigma and Style in Rice. Plant Physiology, 2019, 180(2): 926-936.下载
11. He Y, Zhu M, Wang L, Wu J, Wang Q, Wang R, Zhao Y*. Programmed Self-Elimination of the CRISPR/Cas9 Construct Greatly Accelerates the Isolation of Edited and Transgene-Free Rice Plants. Molecular Plant, 2018, 11(9): 1210-1213.下载
12. He Y*#, Zhang T#, Sun H, Zhan H, Zhao Y*. A reporter for noninvasively monitoring gene expression and plant transformation. Horticulture Research, 2020, 7: 152. (Faculty Opinions 推荐论文，首届江苏省科技期刊优秀论文) 下载 RUBY电子画廊展示
13. He Y, Zhu M, Wang L, Wu J, Wang Q, Wang R, ZhaoY*. Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants. Rice Science, 2019, 26(2): 109-117.下载
14. He Y*#, Zhu M#, Wu J#, Ouyang L, Wang R, Sun H, Yan L, Wang L, Xu M, Zhan H, Zhao Y. Repurposing of anthocyanin biosynthesis for plant transformation and genome editing. Frontiers in Genome Editing, 2020, 2: 607982.下载
15. He Y, Zhao Y*. Technological breakthroughs in generating transgene-free and genetically stable CRISPR-edited plants. aBIOTECH, 2020, 1(1): 88-96. 下载
16. He Y, Zhang T, Yang N, Xu M, Yan L, Wang L, Wang R, Zhao Y*. Self-cleaving ribozymes enable the production of guide RNAs from unlimited choices of promoters for CRISPR/Cas9 mediated genome editing. Journal of Genetics and Genomics, 2017, 44(9): 469-472.下载
17. He Y, Wang R, Dai X, Zhao Y*. On Improving CRISPR for Editing Plant Genes: Ribozyme-Mediated Guide RNA Production and Fluorescence-Based Technology for Isolating Transgene-Free Mutants Generated by CRISPR. Progress in Molecular Biology and Translational Science, 2017, 149: 151-166.下载
18. He Y, Zhao Y*. A key link between jasmonic acid signaling and auxin biosynthesis. Science China Life Sciences, 2015, 58(3): 311-312.下载
19. Wang Y, WeiS, He Y, Yan L, Wang R, Zhao Y*. Synergistic roles of LAX1 and FZP in the development of rice sterile lemma. Crop Journal, 2020, 8(1):16-25. 下载
20. Li S, LiJ, He Y, Xu M, Zhang J, Du W, Zhao Y*, Xia L*. Precise gene replacement in rice by RNA transcript-templated homologous recombination. Nature Biotechnology, 2019, 37(4): 445-450.下载
21. Sun Y, Zhang X, Wu C, He Y, Ma Y, Hou H, Guo X, Du W, Zhao Y*, Xia L*. Engineering Herbicide-Resistant Rice Plants through CRISPR/Cas9-Mediated Homologous Recombination of Acetolactate Synthase. Molecular Plant, 2016, 9(4): 628-631.下载
文件上传中...
