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Stage Ⅰ (before graduation): emphasis on fundamental researchMy research experience began with my PhD project, during which I studied theexpression and function of haloarchaeal hsp70 gene. The nucleotides flanking the startcodon in hsp70 mRNAs with very short 5'-UTRs were mutated, and theircorresponding influences on gene expression (including transcript, translation leveland translational efficiency) were analyzed. Moreover, three genes (grpE, hsp70 andhsp40) of the Hsp70 chaperone machine in Hfx. volcanii were cloned, deleted andwell characterized to analyze their function on the survival and growth of haloarchaeaunder the conditions of high temperature and low salinity.
Stage Ⅱ (after work): emphasis on applied research, especially microbialconversion of oilcrops-based agricultural byproducts to producehigh-value-added metabolites. Following my PhD I moved to Oil Crops Research Institute of Chinese Academy ofAgricultural Science, to work with Professor Fenghong Huang on metabolism ofmicrobial oils, polyunsaturated fatty acids and iturin A, and microbial conversion ofoilcrops-based agricultural byproduct. During this period, several important genes andenzymes were identified and analyzed. In addition, recombinant bacteria andoleaginous yeasts were constructed and demonstrated to produce high levels of fattyacids. Furthermore, agricultural byproducts including rapeseed meal, soybean mealpeanut meal, sesame meal, were assessed. Rapeseed meal fermentation model foriturin A production by Bacillus amyloliquefaciens was established, and the specificroles of different rapeseed meal components on iturin A production were analyzed. Many promising strategies were attempted to achieve full utilization of rapeseed mealfor the production of high-value-added metabolites.
StageIII (as a visiting scholar in Imperial College London): combination offundamental and applied research by Synthetic Biology and Synthetic MicrobialCommunities. This work is aiming to produce those valuable chemicals sustainably by creatingcollaborative communities of yeast with marine cyanobacteria – a microbe growingfrom CO2 and sunlight in seawater or wastewater. A cyanobacterium Synechococcuswas engineered for the production and secretion of sucrose at first. This opensavenues for sugar production in remote places from seawater or wastewater. Afterengineering Yarrowia lipolytica for sucrose consumption and optimising the mediacomposition and salinity, it was shown that the growth of Y. lipolytica can besupported by the sucrose produced by Synechococcus as the sole carbon source. Different yeast producers (including nootkatone, β-caryophyllene, parthenolide andcarotene) can be introduced into the community with cyanobacteria allowing forgrowth and potentially bioproduction from sunlight and CO2, even in placesunsuitable for traditional agriculture thus not competing for agricultural land withfood and feed sources.
项目名称 | 项目来源 | 经费 | 年限 |
菜粕多酚调控裂殖壶菌合成DHA的作用机制研究（主持） | 国家自然科学基金 32001620 | 24 | 2021.01-2023.12 |
湖北省青年英才开发计划（独立） | 中共省委组织部 | 5 | 2019.12-2022.12 |
菜籽饼粕对于解淀粉芽孢杆菌发酵产伊枯草菌素A的应用研究（主持） | 油料所科技成果转化项目 | 30 | 2019.01-2021.12 |
菜籽饼粕中不同组分对于解淀粉芽孢杆菌CX-20发酵产Iturin A的作用机制研究 （主持） | 湖北省自然科学基金 2019CFB378 | 3 | 2019.01-2020.12 |
高附加值油脂的生物合成及代谢调控（参加） | 中国农科院-澳大利亚联邦科工组织国际合作 CAAS-XTCX2018020-7 | 30 | 2018.01-2020.12 |
油料基生物转化关键技术研究（参加） | 农业农村部农业技术试验示范与服务支持项目--农产品加工 | 25 | 2017.01-2017.12 |
利用微生物工程高效降解菜粕抗营养因子和高值化转化技术研究（参加） | 国家“十三五”重大专项子课题 2016YFD050120901 | 70 | 2016.09-2020.12 |
油料基副产物综合利用（参加） | 湖北邦之得牧业有限公司横向 | 10 | 2017 |
高产核酸菌株的改良（参加） | 湖北海宜科技生物有限公司横向 | 10 | 2018 |
1. Huimin Zhang, Zhenxia Xu, Wenchao Chen, Fenghong Huang, ShouwenChen, Xu Wang, Chen Yang. Algal oil alleviates antibiotic-induced intestinalinflammation by regulating gut microbiota and repairing intestinal barrier. FrontNutr. 2023; 9:1081717.
2. Chen Yang, Zhao Zhang, Liang Liu, Yang Li, Xuyan Dong, Wenchao Chen*. Fabrication of soy protein isolate/κ-carrageenan hydrogels for release control ofhydrophilic compounds: Flax lignans. International Journal of BiologicalMacromolecules. 2022; 223:821-829.
3. Xiahui Hao#, Wenchao Chen#, Alberto Amato, Juliette Jouhet, Eric Maréchal, Daniel Moog, Hanhua Hu, Hu Jin, Lingjie You, Fenghong Huang, MarkMoosburner, Andrew E. Allen and Yangmin Gong. Multiplex CRISPR/Cas9editing of the long-chain acyl-CoA synthetase family in the diatomPhaeodactylum tricornutum reveals that mitochondrial ptACSL3 is involved inthe synthesis of storage lipids. New Phytol. 2022; 233(4):1797-1812.
4. Meng Wang, Chen Yang, Jean Marie François, Xia Wan, Qianchun Deng, Danyang Feng, Shiyu Deng, Shouwen Chen, Fenghong Huang, Wenchao Chen*, Yangmin Gong. A two-step strategy for high-value-added utilization of rapeseedmeal by concurrent improvement of phenolic extraction and protein conversionfor microbial iturin A production. Front Bioeng Biotech. 2021; 9:735714.
5. Chen Yang, Zhixian Qiao, Zhenxia Xu, Xu Wang, Qianchun Deng*, WenchaoChen*, Fenghong Huang. Algal Oil Rich in Docosahexaenoic Acid AlleviatesIntestinal Inflammation Induced by Antibiotics Associated with the Modulationof the Gut Microbiome and Metabolome. J Agric Food Chem. 2021; 69(32):9124-9136.
6. Zhao Zhang#, Wenchao Chen#, Xin Zhou, Qianchun Deng, Xuyan Dong, ChenYang, Fenghong Huang. Astaxanthin-loaded emulsion gels stabilized by Maillardreaction products of whey protein and flaxseed gum: Physicochemicalcharacterization and in vitro digestibility. Food Res Int. 2021; 144:110321.
7. Xia Wan, Xue-Rong Zhou, Gabriel Moncalian, Lin Su, Wen-ChaoChen, Hang-Zhi Zhu, Dan Chen, Yang-Min Gong, Feng-HongHuang, Qian-Chun Deng. Reprogramming microorganisms for the biosynthesisof astaxanthin via metabolic engineering. Prog Lipid Res. 2021; 81:101083.
8. Wenchao Chen, Meng Wang, Yangmin Gong, Qianchun Deng, MingmingZheng, Shouwen Chen, Xia Wan, Chen Yang, Fenghong Huang. Theunconventional adverse effects of fungal pretreatment on iturin A fermentation byBacillus amyloliquefaciens CX-20. Microb. Biotechnol. 2021; 14:587-599.
9. Zhenxia Xu#, Wenchao Chen#, Qianchun Deng, Qingde Huang, Xu Wang, ChenYang, Fenghong Huang. Flaxseed oligosaccharides alleviate DSS-induced colitisthrough modulation of gut microbiota and repair of the intestinal barrier in mice. Food Funct. 2020; 11(9):8077-8088.
10. Lian Wang#, Shan Jiang#, Wen-Chao Chen#, Xue-Rong Zhou, Ting-XuanHuang, Feng-Hong Huang, Xia Wan. The Phospholipid:DiacylglycerolAcyltransferase-Mediated Acyl-Coenzyme A-Independent Pathway EfficientlyDiverts Fatty Acid Flux from Phospholipid into Triacylglycerol in Escherichiacoli. Appl Environ Microbiol. 2020; 86(18):e00999-20.
11. Chen Yang, Chao Hu, Hao Zhang, Wenchao Chen, Qianchun Deng, HuTang, Fenghong Huang. Optimation for preparation of oligosaccharides fromflaxseed gum and evaluation of antioxidant and antitumor activities in vitro. Int JBiol Macromol. 2020; 153:1107-1116.
12. Wenchao Chen, Xuli Ma, Xiuzhen Wang, Shouwen Chen, Anna Rogiewicz, Bogdan Slominski, Xia Wan*, Fenghong Huang*. Establishment ofa rapeseed meal fermentation model for iturin A production by Bacillusamyloliquefaciens CX-20. Microb Biotechnol. 2019; 12(6):1417-1429.
13. Wenchao Chen, Xuan Li, Xuli Ma, Shouwen Chen, Yanping Kang, MinminYang, Fenghong Huang, Xia Wan*. Simultaneous hydrolysis with lipase andfermentation of rapeseed cake for iturin A production by Bacillusamyloliquefaciens CX-20. BMC Biotechnol. 2019; 19(1):98.
14. Xuli Ma, Xuan Li, Fan Chen, Wenchao Chen*, Xia Wan, Fenghong Huang. Isolation and characterization of melanin derived from rapeseed meal. Oil CropScience. 2019; 4(1):24-31.
15. Wenchao Chen, Meng Wang, Qi Zhou, Qianchun Deng, Mingming Zheng, ChenYang*, Xia Wan, Fenghong Huang. Research progress of vegetable oils and theirderivatives in microbial fermentation for production of high-value-addedmetabolites. Oil Crop Science. 2019; 4 (4): 208-227.
16. Chen Yang, Zhenxia Xu, Zhou Xin, Qianchun Deng, Wenchao Chen*, FenghongHuang. Dietary flaxseed oil regulate lipid metabolism associated with modulationof gut microbiota in high-fat-diet fed mice. Oil Crop Science. 2019; 4(4):195-207.
17. Xiuhong Yue#, Wenchao Chen#, Zhiming Wang, Pengyang Liu, Xiangyu Li, Chubin Lin, Shuhuan Lu, Fenghong Huang, Xia Wan*. Lipid Distribution Patternand Transcriptomic Insights Revealed the Potential Mechanism ofDocosahexaenoic Acid Traffics in Schizochytrium sp. A-2. Journal of Agriculturaland Food Chemistry. 2019; 67:9683.
18. Kang Xiao, Xiuhong Yue, Wenchao Chen, Xuerong Zhou, Lian Wang, Lin Xu, Fenghong Huang, Xia Wan*. Metabolic engineering for enhanced medium chainomega hydroxy fatty acid production in Escherichia coli. Front Microbiol. 2018;9:139.
19. 王萌, 柴保中, 奚钊, 万楚筠, 李念祖, 陈文超*, 万霞, 黄凤洪. 固体发酵技术在油料饼粕合成高附加值微生物代谢产物中的研究进展. 中国油料作物学报. 2021; 43(2):322-337.
20. 王萌, 奚钊, 万楚筠, 陈文超*, 万霞, 黄凤洪. 微生物发酵在菜籽饼粕饲用品质改良中的应用研究进展. 中国油料作物学报. 2020; 42(2):313-324.
PATENTS
1. 一种油菜籽副产物的综合利用方法. 陈文超; 刘佩; 龚阳敏; 丁小霞; 王萌. 2022-12-02, 中国, 202211539740.3
2. 一种富硒酵母菌、益生菌复合菌液以及发酵豆粕饲料. 龚阳敏; 张毅; 胡传炯; 陈文超; 万霞; 郑明明; 黄凤洪. 2022-02-16, 中国,ZL202210140287.2.
3. 一种真核工程菌株及其制备方法和应用.万霞; 陈文超; 黄凤洪; 邓乾春;朱航志; 蒋珊. 2019-09-25, 中国, CN201910911559.2.
4. 一种原核工程菌株及其制备方法和应用. 万霞; 陈文超; 黄凤洪; 邓乾春;朱航志; 蒋珊. 2019-09-25, 中国, CN201910911590.6.
5. 一种稳定高效水解油料粕的菌株及其应用. 陈文超; 万霞; 李旋; 马旭丽;黄凤洪. 2018-11-26, 中国, ZL201811416135.0.
6. 一种极端酸、碱水解法在芽孢杆菌发酵油料粕生产伊枯草菌素 A 中的应用及其方法. 陈文超; 万霞; 李旋; 马旭丽; 黄凤洪; 郑明明. 2018-11-26, 中国, CN201811416625.0.
7. 一种分析油料粕中矿物质在芽孢杆菌发酵生产伊枯草菌素 A 中作用的方法. 陈文超; 万霞; 李旋; 马旭丽; 黄凤洪; 郑明明. 2018-11-26, 中国,CN201811417088.1.
8. 一种菜粕中提取黑色素的方法及其方法所获得的沉淀物的应用. 陈文超; 马旭丽; 万霞; 黄凤洪; 李旋. 2018-06-29, 中国, ZL201810693287.9.
9. 一种双低菜籽饼和玉米秸秆混合青贮方法. 李念祖; 万霞; 向欣; 陈文超;黄凤洪; 王友海. 2018-04-10, 中国, CN201810323423.5.
10. 一种具有蜡酯合成活性和甘油三酯合成活性的双功能酶及其编码基因和应用. 龚阳敏; 万霞; 张南南; 黄凤洪; 陈文超. 2017-04-27, 中国,ZL201710286855.9.
11. 一种产二十碳五烯酸的菌株及其应用. 万霞; 彭云峰; 陈文超. 2016-07-28, 中国, ZL201610607139.1.
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