本团队重点关注我国集约化及智能养殖环境下猪禽呼吸道、肠道健康，分析猪禽黏膜系统微环境中病原生态学及生物学特征，剖析猪禽黏膜系统疫病的病原体组成、动态变化及致病规律；解析猪禽黏膜感染与免疫应答机制，探讨黏膜上皮细胞与免疫细胞在免疫应答中的相互作用，阐明猪禽黏膜系统病原感染及免疫应答的分子基础及调控网络；基于猪禽呼吸道、消化道等黏膜系统病原学状况及黏膜免疫应答规律，研发、创制高效猪禽黏膜疫苗、佐剂等疫病防控产品，开发新型黏膜免疫策略及配套检测方法。

1.     项目名称：猪肠道类器官3D培养系统的建立及其在PEDV感染及免疫应答研究中的应用

项目编号：1610312020020

项目来源：基本科研业务费，59万元（直接经费）

执行期限：2020年1月至2023年12月

2.     项目名称：PEDV感染通过诱生肠道微环境TGF-β1促进B细胞凋亡的分子机制

项目编号：31972689

项目来源：国家自然基金面上项目，59万元（直接经费）

执行期限：2020年1月至2022年12月

3.     项目名称：新发与再现畜禽重大疫病的致病与免疫机制研究

项目编号：2016YFD0500103

项目来源：十三五重点研发，140万元

执行期限：2016年6月至2020年12月

4.     项目名称：猪流行性腹泻病毒诱导T细胞依赖性sIgA+ B细胞分化机制

项目编号：31572498

项目来源：国家自然基金面上项目，76.4万元

执行期限：2016年1月至2019年12月

5.      项目名称：“青年英才计划”择优支持启动基金

项目编号：CAASQNYC-KYYJ-40

项目来源：中国农业科学院，300万元

执行期限：2015年10月至2019年9月

PUBLICATIONS (PEER REVIEWED)

1.      Zhang N,Shi H, Yan M, Liu G. IFIT5 Negatively Regulates the Type I IFN Pathwayby Disrupting TBK1-IKKε-IRF3 Signalosome and Degrading IRF3 and IKKε. Journal of Immunology. 2021, 206(9): 2184-2197.

2.     Aryal M, Liu G. Porcine Bocavirus:A 10-Year History since Its Discovery. VirologicaSinica. 2021 Apr 28:1-12.

3.     Chen J, Cui Y, Wang Z, Liu G*. Identification and characterization of PEDVinfection in rat crypt epithelial cells. Veterinary Microbiology, 2020, 249(10): 108848

4.     Li Y#, Ning Yang N#,Chen J, Huang X, Zhang N, Yang S, Liu G, LiuG*. Next-generation porcine intestinal organoids: an apical-outorganoid model for swine enteric virus infection and immune responseinvestigations. Journal of Virology,2020, 94(21): e01006-20.

5.     Yang S, Li Y, Wang B, Yang N, Huang X, Chen Q, Geng S, Zhou Y, Shi H, Wang L, Brugman S, Savelkoul H, Liu G*. Acute porcine epidemic diarrhea virus infection reshapes theintestinal microbiota. Virology. 2020, 548(9), 200-212.

6.     Liu G, Wang B, Chen Q, Li Y, LiB, Yang N, Yang S, Liu G*.Interleukin (IL)-21 Promotes the Differentiation of IgA-Producing Plasma Cellsin Porcine Peyer's Patches via the JAK-STAT Signaling Pathway. Frontiers in Immunology, 2020, 11: 1303.DOI: 10.3389/fimmu.2020.01303

7.     Chen J, Jin L, Wang Z, Wang L,Chen Q, Cui Y, Liu G*.N6-methyladenosine regulates PEDV replication and host gene expression. Virology. 2020, 548(9): 59-72

8.     Yan M#, Huang J#,Chen J, Yang W, Liu G*.Preparation, identification, and functional analysis of monoclonal antibodiesagainst atypical porcine pestivirus NS3 protein. Journal of Veterinary Diagnostic Investigation, 2020, 32(5):695-699.

9.     Fu Y, Li B, Liu G*. Rapid and efficientdetection methods of pathogenic swine enteric coronaviruses. Applied Microbiology and Biotechnology.2020, 104(14): 6091-6100.

10.  Ding G#, Fu Y#, Li B, Chen J, Wang J, Yin B,Sha W, Liu G*. Developmentof a multiplex RT-PCR for the detection of major diarrhoeal viruses in pigherds in China. Transboundary Emerging Diseases.2020, 67(2):678-685.

11.  Huang X, Chen J, Yao G, Guo Q, Wang J, Liu G*. A TaqMan-probe-based multiplex real-time RT-qPCR forsimultaneous detection of porcine enteric coronaviruses. Applied Microbiology and Biotechnology. 2019, 103(12): 4943-4952.

12.  Chen J, Jin L, Yan M, Yang Z, Wang H, Geng S, Gong Z, Liu G*. Serum Exosomes fromNewborn Piglets Restrict Porcine Epidemic Diarrhea Virus Infection. Journal of Proteome Research. 2019, 18(5):1939-1947.

13.  Yang W, Chen W, Huang J, Jin L, Zhou Y, Chen J, Zhang N, Wu D, SunE, Liu G*. Generation,identification, and functional analysis of monoclonal antibodies againstporcine epidemic diarrhea virus nucleocapsid. Applied Microbiology and Biotechnology. 2019, 103(9): 3705-3714.

14.  Liu G, Zhang F, Wang R, London SD, London L*. Salivary glandimmunization via Wharton's duct activates differential T-cellresponses within the salivary gland immune system. FASEB Journal. 2019, 33(5):6011-6022.

15.  Chen J, Wang H, Jin L, Wang L, Huang X, Chen W, Yan M, Liu G*. Profile analysis ofcircRNAs induced by porcine endemic diarrhea virus infection in porcineintestinal epithelial cells. Virology.2019, 527:169-179.

16.  Chen J, Zhang C, Zhang N, LiuG*. Porcine endemic diarrhea virus infection regulates long noncodingRNA expression. Virology. 2019, 527:89-97.

17. Fu Y, Tong J, Meng F, HoeltigD, Liu G, Yin X, Herrler G*.Ciliostasis of airway epithelial cells facilitates influenza A virus infection.Veterinary Research. 2018, 49(1):65.

18. Chen J, Zhang C, Liu Y, Liu G*, 2017. Super-oxidizedwater inactivates major viruses circulating in swine farms. Journal of Virological Methods.242:27-29

19. Meng Q*, Liu G*, Liu Y, Deng X, Wang W, Xu K, Zheng X, Zhang D, PangH, Chen H, 2015. A broad protection provided by matrix protein 2 (M2) of avianinfluenza virus. Vaccine, 33(31): 3758-3765(* Co-first author)

20.  Liu G, Zhang F, Wang R, London L,London S, 2014. Protective MCMV Immunity by Vaccination of the Salivary Glandvia Wharton’s Duct: Replication-deficient Recombinant Adenovirus ExpressingIndividual MCMV Genes Elicit Protection Similar to that of MCMV. FASEB Journal, 28(4):1698-1710.

21.  Liu G, Zhang F, Shi J, Tian G, Chen H, Yu K Meng Q, 2013. A Subunit Vaccine Candidate Derived from aClassic H5N1 Avian Influenza Virus in China Protects Fowls and BALB/c Mice fromLethal Challenge, Vaccine, 31(46): 5398-5404.

22.  Wang H, Deng X, Zhou T, Wang C, Hou Y, Jiang H, Liu G*,2013. The in vitro immunomodulatory activity of a polysaccharide isolated fromKadsura marmorata. Carbohydrate Polymers. 97: 710-715 (* Corresponding author)

23.  Avasarala S., Zhang F,Liu G, Wang R, London S, London L, 2013. Curcumin Modulates the InflammatoryResponse and Inhibits Subsequent Fibrosis in a Mouse Model of Viral-inducedAcute Respiratory Distress Syndrome. Plos One, 8(2):e57285.

24.  Liu G, Wang Q, Liu N, Tong T, Xiao Y,Liu S, Wu D, 2012. Infectious Bronchitis Virus Nucleoprotein Specific CTL Response isGenerated Prior to Serum IgG. Veterinary Immunology and Immunopathology. 148 (3-4): 353-358.

25.  Kahan SM, Liu G,Reinhard MK, Hsu CC, Livingston RS, Karst SM, 2011. Comparative murinenorovirus studies reveal a lack of correlation between intestinal virus titersand enteric pathology. Virology 421: 202-210.

26.  Zhang W, Lin Y, Bai Y,Tong T, Wang Q, Liu N, Liu G, Xiao Y, Yang T, Bu Z, Tong G, Wu D,2011. Identification of CD8+ cytotoxic T lymphocyte epitopes from porcinereproductive and respiratory syndrome virus matrix protein in BALB/c mice. Virology Journal, 8(1): 263.

27.  Liu G, Jia Y, Kahan MS, Karst MS, 2009. Primary high-dose murine norovirus 1 infection fails to protect fromsecondary challenge with homologous virus. Journalof Virology, 83: 6963-6968.

28.  Liu G, Wang Q, Xiao Y, Tong T, Bai Y,Liu S, Wu D, 2008. Construction and functional test of BF2-peptide tetramer. Veterinary Immunology andImmunopathology. 122 (1-2):1-7.

29.  Changotra H, Jia Y, Moore N.T, LiuG, Kahan MS, Sosnovtsev VS, Karst MS, 2009. Type I and type II interferon inhibit the translation of murine norovirusproteins. Journal of Virology, 83:5683-5692.

30.  Tong T., Bai Y., Liu G.,Wang Q., Zhang W., Xiao Y., Xu S., Liu N., Yang T., Wu D, 2010, Expression,purification and monoclonal antibodies preparation of recombinant equine matureinterleukin-18. Veterinary Immunology andImmunopathology, 136: 194-200.

31.  Bai Y, Tong T, Liu G, Chen W, Zhang W, Wang Q, Yang T, Bu Z, Wu D, 2010. Expressionof biologically active recombinant equine interferon-g in Escherichia coli. ComparativeImmunology Microbiology Infectious Diseases, 33: 333–342.

32.  Xiao Y, Meng Q, Yin X,Guan Y, Liu Y, Li C, Wang M, Liu G,Tong T, Wang L, Kong X, Wu D, 2008. Pathology of palm civets experimentallyinfected by Severe Acute Respiratory Syndrome Coronavirus. Journal of ComparativePathology 138: 171-179.

PATENTS

1.     2019, PatentNo. ZL201910482822.0, A PCR assay for detection of class switch recombinationof swine IgA+ B cells.

2.     2018, ApplicationNo. 201811007934.2, A multiplex RT-qPCR for detection of swine entericcoronaviruses. (Under review)

3.     2016, PatentNo. ZL201610017701.2, A multiplex RT-PCR for inclusive detection of 5 diarrhealviruses in pigs.

4.     2006,Patent No. ZL200410096238.5. The Method of Preparation for H5N1 Avian InfluenzaVirus Gene Engineering Subunit Vaccine.

5.      2009, Patent No. ZL200710152068.1. The method toconstruct Chicken Major Histocompatibility Complex/peptide Tetramer.