主持的课题

1.      国家自然科学基金青年项目，项目批准号：21807111，项目名称：定向进化调控Baeyer-Villiger单加氧酶TmCHMO的区域选择性及机制解析，2019.01-2021.12（25万）.

2.      国家自然科学基金面上项目，项目批准号：31972327，项目名称：解析嗜线虫致病杆菌CB6中抗菌活性物质Xcn1转化的分子机制，2020.01-2023.12（57万）.

3.     中国农业科学院青年英才“A”启动基金（100万）.

4.     中国农业科学院重大科研任务“小麦藏粮于技”子任务“小麦病虫害绿色防控集成技术”，项目批准号：CAAS-ZDRW202002 （30万/年）.

5.     自治区区域协同创新专项（科技援疆计划），2021.01-2023.12 （60万）（疆外单位负责人）；

参加的课题

1.      中国农业科学院创新工程协同创新任务，项目批准号：CAAS-XTCX2019024，项目名称：农产品质量安全速测产品创制与示范应用，2019.01-2022.12（2230万）

2.      中国农业科学院基本科研业务费专项院级统筹项目（30万）；

3.     自治区区域协同创新专项（科技援疆计划），2019.01-2021.12 （40万）；

1.       Zhao Mingqi, Wang Miao*, ZhangXiaoguang, Zhu Yongan, Cao Jing, She Yongxin, Cao Zhen, Li Guangyue*,Wang Jing*，Abd  A. M. Recognition elements based on themolecular biological techniques for detecting pesticides in food: A review.Critical Reviews in Food Science and Nutrition, 2021,DOI10.1080/10408398.2021.2009762. (IF=11.2)

2.       Youcai Qin, Fenglian Jia,Xiaohui Li, Beibei Li, Jie Ren, Xiufen Yang and Guangyue Li*. Improvingthe Yield of Xenocoumacin 1 by PBAD Promoter Replacement in Xenorhabdusnematophila CB6. Agriculture, 2021, 11: 1251. (IF=2.92)

3.       Li, G#.; Qin, Y#.; Fontaine, N. T#.; Ng Fuk Chong, M.;Maria‐Solano, M. A.; Feixas, F.; Cadet, X. F.; Pandjaitan, R.;Garcia‐Borràs, M.; Cadet, F., Reetz, M. T. Machine Learning EnablesSelection of Epistatic Enzyme Mutants for Stability Against Unfolding andDetrimental Aggregation. ChemBioChem. 2020, 21,1-12. (IF5year =2.751)

4.       李广悦, 杨秀芬. 嗜线虫致病杆菌抗菌代谢产物 Xcn1 的研究进展. 中国生物防治学报 2020,36 (1), 1-8.

5.       段佳琪，李琲琲，任杰，邱德文，李广悦*.一种脱氧雪腐镰刀菌烯醇制备工艺的研究.生物技术通报. 2020, 36(10):1-8.

6.       Dong, Y.; Li, X.; Duan, J.; Qin, Y.; Yang, X.; Ren, J.; Li, G*. Improving the Yield ofXenocoumacin 1 Enabled by In Situ Product Removal. ACS omega. 2020, 5（32）, 20391–20398. (IF5year =2.6)

7.       Jiaqi Duan, Beibei Li,Youcai Qin, Yijie Dong, Jie Ren and GuangyueLi*. Recentprogress in directed evolution of stereoselective monoamine oxidases. Bioresources andBioprocessing [J]. 2019, 6: 1-11. (IF5year =4.76)

8.       Guangyue Li, Yijie Dong, and Manfred T. Reetz*. Can Machine LearningRevolutionize Directed Evolution of Selective Enzymes? Adv. Synth. Catal [J]. 2019, 361, 2377 – 2386. (IF5year =5.029)

9.       Guangyue Li, Marc Garcia-Borràs,Maximilian J. L. J. Fürst, Adriana Ilie, Marco W. Fraaije, K. N. Houk*andManfred T. Reetz*. OverridingTraditional Electronic Effects in Biocatalytic Baeyer-Villiger Reactions byDirected Evolution. JACS [J]. 2018, 140,10464−10472. (IF5year =14.35)

10.   Yijie Dong#, Peiyuan Yao#, Yunfeng Cui, Qiaqing Wu,Dunming Zhu*, Guangyue Li*andManfred T. Reetz*. Manipulating the stereoselectivity of a thermostable alcoholdehydrogenase by directed evolution for efficient asymmetric synthesis ofarylpropanols. Biological Chemistry [J]. 2019，400(3), 313-321.(IF5year =3.2)

11.   Guangyue Li#, Hui Zhang#, Zhoutong Sun, Xinqi Liu and Manfred T. Reetz*. MultiparameterOptimization in Directed Evolution: Engineering Thermostability, Enantioselectivity,and Activity of an Epoxide Hydrolase. ACS Catalysis [J]. 2016, 6(6), 3679−3687. (IF5year =10.945)

12.   Guangyue Li#, Peiyuan Yao#, Rui Gong#, Jinlong Li, Pi Liu，Richard Lonsdale, Qiaqing Wu, Jianping Lin*，Dunming Zhu*and Manfred T. Reetz*. Simultaneous engineering of an enzyme’s entrance tunneland active site: The case of monoamine oxidase MAO-N. Chemical Science [J]. 2017, 8，4093-4099. (IF5year=8.733)

13.   Guangyue Li#, Jie Ren#, Peiyuan Yao,Yitao Duan, Hailing Zhang, Qiaqing Wu, Jinhui Feng, Peter CKLau*, Dunming Zhu*. Deracemization of 2-Methyl-1, 2, 3, 4-tetrahydroquinolineUsing Mutant Cyclohexylamine Oxidase Obtained by Iterative SaturationMutagenesis. ACS Catalysis [J].2014, 4，903-908. (IF5year =9.174)

14.   Guangyue Li, Miguel A. Maria-Solano, AdrianRomero-Rivera, Sílvia Osuna* and Manfred T. Reetz*. Inducing High Activity of aThermophilic Enzyme at Ambient Temperatures by Directed Evolution. Chemical Communications [J]. 2017, 53，9454-9457. (IF5year =6.238)

15.   Guangyue Li and Manfred T. Reetz*. Learning Lessonsfrom Directed Evolution of Stereoselective Enzymes. Org. Chem. Front[J]. 2016. 3, 1350-1358 (IF5year =4.96)

16.   Guangyue Li#, Peiyuan Yao#, Peiqian Cong, Jie Ren, Lei Wang, Jinhui Feng, Peter C.K. Lau, QiaqingWu*,and Dunming Zhu*. New recombinant cyclohexylamine oxidase variants forderacemization of secondary amines by orthogonally assaying designed mutantswith structurally diverse substrates. Scientificreports [J]. 2016，6：24973. (IF5year =4.847)

17.   Guangyue Li, M.J. L. J. Fürst, H. R. Mansouri, A. K. Ressmann, A.Ilie, F. Rudroff, M. D. Mihovilovic*, M.W. Fraaije* and M.T. Reetz*. Manipulatingthe stereoselectivity of the thermostable Baeyer–Villiger monooxygenase TmCHMOby directed evolution. Organic & Biomolecular Chemistry [J]. 2017，15，9824-9829. (IF5year =3.56)

18.   Guangyue Li, Jianbo Wangand Manfred T. Reetz*. Biocatalysts for the pharmaceutical industry generatedby structure-guided directed evolution ofstereoselective enzymes. Bioorganic & MedicinalChemistry [J]. 2017, 7(26), 1241-1251 (IF5year=2.88)

19.   Guangyue Li, Jie Ren,Hiroaki Iwaki, Dalong Zhang, Yoshie Hasegawa, Qiaqing Wu, Jinhui Feng, Peter C.K. Lau* and Dunming Zhu*. Substrate profiling of cyclohexylamine oxidase andits mutants reveals new biocatalytic potential in deracemization of racemicamines. AppliedMicrobiology and Biotechnology [J].2014, 98(4)，1681-1689. (IF5year =3.848)

20.   Guangyue Li, Jie Ren,Qiaqing Wu*, Jinhui Feng, Dunming Zhu*, Yanhe Ma. Identification of a marineNADPH-dependent aldehyde reductase for chemoselective reduction of aldehydes. Journal of molecular catalysis B, Enzymatic[J], 2013, 90，17-22. (IF5year =2.786)

21.   Li GuangYue，Yang XiuFen，Zeng HongMei，Mao JianJun，Liu Hua，Zhang YunHua，Qiu, DeWen* Stable isotope labelled mass spectrometry for quantification of therelative abundances for expressed proteins induced by PeaT1. Science China Life Sciences [J], 2010,53(12)，1410-7. (IF5year=2.88)

22.   Frédéric Cadet, Nicolas Fontaine, Guangyue Li, JoaquinSanchis, Matthieu Ng Fuk Chong,Rudy Pandjaitan, IyanarVetrivel , Bernard Offmann & Manfred T. Reetz. A machine learning approachfor reliable prediction of amino acid interactions and its application in thedirected evolution of enantioselective enzymes. Scientific reports [J]. 2018, 8:16757. (IF5year =4.12)

23.   Zhoutong Sun†, Guangyue Li†, Adriana Ilie†,Manfred T. Reetz *. Exploring the substrate scope of mutants derived from therobust alcohol dehydrogenase TbSADH. Tetrahedron Letters[J]. 2016. 57, 3648-3651.(IF5year =2.02).

24.   Peiyuan Yao, Peiqian Cong, Rui Gong, Jinlong Li, Guangyue Li, Jie Ren, Jinhui Feng,Jianping Lin, Peter C. K. Lau,* Qiaqing Wu,* and Dunming Zhu*.BiocatalyticRoute to Chiral 2-Substituted-1, 2, 3, 4-tetrahydroquinolines Using CyclohexylamineOxidase Muteins. ACS Catalysis[J].2018, 8, 1648-1652. (IF5year =10.945).

25.   Ge Qu, Richard Lonsdale, Peiyuan Yao, Guangyue Li, Beibei Liu, Manfred T.Reetz,* and Zhoutong Sun*. Methodology Development in Directed Evolution:Exploring Options when Applying Triple‐CodeSaturation Mutagenesis. ChemBioChem[J].2018, 19, 239-246. (IF5year =2.751).

26.   Jian-bo Wang, Guangyue Li, Manfred T. Reetz*. Enzymatic site-selectivity enabledby structure-guided directed evolution. Chemical Communications [J]. 2017, 53, 3916-3928. (IF5year =6.238)

27.    Zhoutong Sun, Richard Lonsdale, Adriana Ilie, Guangyue Li, Jiahai Zhou, and Manfred T. Reetz. CatalyticAsymmetric Reduction of Difficult-to-Reduce Ketones:Triple-Code SaturationMutagenesis of an Alcohol Dehydrogenase. ACSCatalysis [J]. 2016, 6(3)，1598−1605. (IF5year =10.945)

28.    Zhoutong Sun, Richard Lonsdale, Lian Wu, Guangyue Li, Aitao Li, Jianbo Wang, Jiahai Zhou, and Manfred T.Reetz. Structure-Guided Triple-Code Saturation Mutagenesis: Efficient Tuning ofthe Stereoselectivity of an Epoxide Hydrolase. ACS Catalysis [J]. 2016, 6， 1590−1597. (IF5year =10.945)

29.   Zhoutong Sun, RichardLonsdale, Guangyue Li and Manfred T.Reetz. Comparing Different Strategies in Directed Evolution of EnzymeStereoselectivity: Single versus Double Code Saturation Mutagenesis. Chembiochem[J]. 2016, 17，1865-1872. (IF5year =2.751)

30.   梁颖博, 李泽, 邱德文, 曾洪梅, 李广悦, 杨秀芬. 本生烟响应蛋白激发子 PevD1 的差异表达基因鉴定与分析. 中国农业科学, 2019，52(21),3794-3805.

31.    刘 权, 李广悦, 曾洪梅, 杨秀芬, 邱德文. 微生物蛋白激发子PeaT1的获得及诱导水稻抗旱性的初步研究. 中国农业科技导报, 2009, 11( 3)， 51- 55.

32.   刘权，李广悦，曾洪梅，杨秀芬，邱德文．蛋白激发子PeaT1的高密度发酵及生理功能检测．生物技术通报，2009，8，162-165．

33.   ZhangLin, Yan Jianpei, Fu Zhenchao, Shi Wenjiong, Ninkuu Vincent, Li Guangyue, YangXiufen, Zeng Hongmei*. FoEG1, a secreted glycoside hydrolase family 12 proteinfrom Fusarium oxysporum, triggers cell death and modulates plant immunity.Molecular Plant Pathology, 2021,22(5):522-538. (IF=5.62)

34.   LiangYingbo, Li Ze, ZhangYi, Meng Fanlu, qiu Dewen, Zeng Hongmei, Li Guangyue, YangXiufen*. Nbnrp1 mediates Verticillium dahliae effector PevD1-triggereddefense responses by regulating sesquiterpenoid phytoalexins biosynthesispathway in Nicotiana benthamiana. gene, 2021, 768:145280. (IF=3.688)

35.   李泽,梁颖博,段佳琪,曾洪梅,李广悦,赵国存,杨秀芬*。蛋白激发子PevD1诱导本生烟植保素辣椒醇的产生和积累。植物保护, 2021,47(01):31-39。