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中国农业科学院农业环境与可持续发展研究所，研究员，博士生导师，青年英才，2018-至今

德国马克思普朗克陆地微生物学研究所，博士后，2013-2018

中国科学院青岛生物能源与过程研究所，助理研究员，2013

韩国光州科学技术院，环境微生物学，博士，2013

中国农业科学院作物科学研究所，生物化学与分子生物学，硕士，2006

研究方向Research Directions

碳氮循环微生物,植物微生物组,烃类物质微生物代谢,农业水土环境

2. 机电结构优化与控制研究内容：在对机电结构进行分析和优化的基础上，运用控制理论进行结构参数的调整，使结构性能满足设计要求。1. 仿生结构材料拓扑优化设计, 仿生机械设计研究内容：以仿生结构为研究对象，运用连续体结构拓扑优化设计理论和方法，对多相仿生结构(机构)材料进行2. 机电结构优化与控制研究内容：在对机电结构进行分析和优化的基础上，运用控制理论进行结构参数的调整，使结构性能满足设计要求。1. 仿生结构材料拓扑优化设计, 仿生机械设计研究内容：以仿生结构为研究对象，运用连续体结构拓扑优化设计理论和方法，对多相仿生结构(机构)材料进行整体布局设计。整体布局设计。

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招生信息

环发所

硕士研究生

序号
专业
年份

(095132)资源利用与植物保护(非全日制)

2022

博士研究生

序号
专业
年份

1

(0903Z1)★农业水资源与环境

2022

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科研项目

主持参加国家自然科学基金、农业农村部项目，青年英才计划、德国DFG、韩国农村振兴厅等项目。

研究成果

1. Yanshuo Pan, Surina Borjigin, Ye Liu, Hongzhe Wang,Yanan Wang, Yingchun Wu, Guangfei Hao, Daozhi Gong, Qingwen Yang, ZengchaoGeng, Yuzhong Li, Dongfei Han^†.2022. Role of key-stone microbial taxa in algae amended soil for mediatingnitrogen transformation. Science ofTotal Env. doi:10.1016/j.scitotenv.2022.153547.
2. Xingjie Wu, Christopher Rensing, Dongfei Han, Ke-Qing Xiao, Werner Liesack, Yuexiu Dai, ZhixiTang, Jingjing Peng, Zhenling Cui, Fusuo Zhang. 2022. Genome-resolvedmetagenomics reveals distinct phosphorous acquisition strategies between soilmicrobiomes. mSystems, 7(1):e01107-21.
3. Wenbo Fu, Yanshuo Pan, Yuhua Shi, Daozhi Gong, YuzhongLi,Yuhua Shi, Guangfei Hao, Dongfei Han^†. 2022. Rootmorphogenesis of Arabidopsis thalianatuned by plant growth promoting Streptomycesisolated from root-associated soil of Artemisiaannua. Frontiers Plant Sci. 12: 802737.
4. Hao Cao, Xinyu Zhang, Shuangyan Wang, JiadingLiu, Dongfei Han, Baisuo Zhao,Haisheng Wang. 2021. Insights into mechanism of the naphthalene-enhancedbiodegradation of phenanthrene by Pseudomonassp. SL-6 based on omics analysis. Frontiers Microbiol. fmicb.2021.761216.
5. Yuhua Shi, Yanshuo Pan, LiXiang, Zhihui Zhu,Wenbo Fu, Guangfei Hao, Zengchao Geng, Shilin Chen, YuzhongLi^†, Dongfei Han^†. 2021. Assembly ofrhizosphere microbial communities in Artemisiaannua: recruitment of plant growth-promoting microorganisms andinter-kingdom interactions between bacteria and fungi. Plant and Soil, s11104-021-04829-9.
6. Yingchun Wu, Guangfei Hao,and Dongfei Han^†. (2021). Sample preparationfor bacterial transcriptome analysis. Bioprotocol,e2003650.
7. Jintong Zhao, Xiaoqing Liu,Dongfei Han^†, and JianTian^†. 2021. Screening method of functional microorganismsinteracting with rice roots. Bioprotocol,e2003638.
8. Yanshuo Han, Jian Tian,Yuzhong Li, Hor-Gil Hur, Dongfei Han^†. 2020. Complete genome sequence of aplant-derived phenylpropanoid-degrading bacterium, Pseudomonas putidaJYR-1, Microbiol. Resour. Announc. 9:e01152-19.
9. Ye Yao, Bo Fu, DongfeiHan, Yan Zhang, He Liu. 2020. Formate-dependentacetogenic utilization of glucose by the fecal acetogen Clostridium bovifaecis. Appl. Environ. Microbiol. 86(23):e01870-20.
10. Nan Wang, Feifei Guan,Xiang Lv, Dongfei Han,Ningfeng Wu, Xiaole Xia, Jian Tian. 2020. Enhancing secretion of polyethyleneterephthalate hydrolase PETase in Bacillus subtilis WB600 mediated by the SPamy signal peptide, Letters Appl. Microbiol. 71(3):235-241
11. Dongfei Han, Svetlana N. Dedysh, Werner Liesack. 2018. Unusual genomic traits suggest Methylocystis bryophila S285 to be welladapted for life in peatlands. Genome Biol. Evol. 10(2): 623-628.
12. Dongfei Han, Hannes Link, Werner Liesack. 2017. Response of Methylocystissp. strain SC2 to salt stress: physiology, global transcriptome, and amino acid profiles. Appl. Environ.Microbiol. 83: e00866-17. (Spotlight selected by editor)
13. YueSun*, Li Ma*, Dongfei Han*, Lei Du, Fengxia Qi, Wei Zhang,Jingran Sun, Shan Huang, Eung-Soo Kim, Shengying Li. 2017. Invitro reconstitutionof the cyclosporine specific P450 hydroxylases using heterologous redox partnerproteins, J. Ind. Microbiol. Biotechnol. 44: 161-166.
14. Xianliang Zheng, Bo Fang, Dongfei Han, Wenxia Yang, FeifeiQi, Hui Chen, Shengying Li. 2016. Improving the secretory expression of an α-galactosidasefrom Aspergillus niger in Pichia pastoris, PLoS ONE 11(8): e0161529.
15. Ruirui Feng, Bo Liang,Chuantao Hou, Dongfei Han,Qiaolin Lang, Lihui Han, Aihua Liu. 2016. Rational design of xylosedehydrogenase for improved thermostability and its application in developmentof efficient enzymatic biofuel cell, Enzyme Microb. Technol. 84: 78-85.
16. Jianxia Song*, Bo Liang*, Dongfei Han*, Xiangjiang Tang,Qiaolin Lang, Ruirui Feng, Lihui Han, Aihua Liu. 2015. Bacterial cell-surfacedisplaying of thermo-tolerant glutamate dehydrogenase and its application inL-glutamate assay, Enzyme Microb. Technol. 70: 72-78.
17. Hyunji Lee, Jiyoung Park,Chaewon Jung, Dongfei Han,Jiyoung Seo, Joong-Hoon Ahn, Youhoon Chong, Hor-Gil Hur. 2015. Enhancement ofthe catalytic activity of ferulic acid decarboxylase from Enterobacter sp. Px6-4 through random and site-directed mutagenesis,Appl. Microbiol. Biotechnol. 99: 9473-9481.
18. Xiangjiang Tang, TingtingZhang, Bo Liang, Dongfei Han,Lingxing Zeng, Cheng Zheng, Tie Li, Mingdeng Wei, Aihua Liu. 2014. Sensitive electrochemicalmicrobial biosensor for p-nitrophenylorganophosphates based on electrodemodified with cell surface-displayed organophosphorus hydrolase and orderedmesopore carbons, Biosens. Bioelectron. 60: 137-142.
19. Dongfei Han, Michael J. Sadowsky, Hor-Gil Hur. 2013. Characterization of a self-sufficient trans-anethole oxygenase by Pseudomonas putida JYR-1. PLoS ONE 8(9): e73350.
20. Dongfei Han, Ji-Young Ryu, Hyunji Lee, Hor-Gil Hur. 2013. Bacterial biotransformation ofphenylpropanoid compounds for producing flavor and fragrance compounds. App. Biol. Chem. 56:125-133.
21. Dongfei Han, Somwang Kurusarttra,Ji-Young Ryu, Robert A. Kanaly, Hor-Gil Hur. 2012. Production of natural fragrance aromatic acids by coexpressingtrans-anethole oxygenase and p-anisaldehyde dehydrogenase genes of Pseudomonas putida JYR-1 in Escherichia coli. J. Agric. Food Chem. 60: 11972-11979.
22. Dongfei Han, Ji-Young Ryu, Robert A. Kanaly Hor-Gil Hur. 2012. Isolation of agene responsible for the oxidation of trans-anetholeto para-anisaldehyde by Pseudomonas putida JYR-1 andits expression in E. coli. Appl. Environ. Microbiol. 78:5238-5246.

专著

1. 韩东飞等.2021. 《微生物组实验手册》，Bio-Protocol

标准制定

1. 韩东飞，付文博，郝光飞等. 2021. 根际促生菌剂标准，农业行业标准
2. 韩东飞，王宏哲，杨柳青等. 2021. 硝化抑制剂施用效果及环境评价技术规范，农业行业标准

专利

1. 韩东飞，王宏哲.2022. 一种具有降解聚乙烯功能的戈登氏菌及其应用,专利号202210029072.3
2. 韩东飞，付文博. 2021. 一种促进植物根部生长的放线菌及其应用,专利号202111234302.1
3. Hor-Gil Hur, Dongfei Han, A New-type trans-AnetholeOxygenase. Korean Patent No.10-2011-002-367 (2011.03.08)

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