团队瞄准国家战略需求，紧密围绕“四个面向”的战略目标，聚焦严重危害养殖业健康发展重要细菌病的科学问题，在科学理论突破、技术创新和产品创制等方面开展研究工作，为我国养殖业健康发展和公共卫生安全提供重要的保障。自2005年以来，先后承担973计划、863计划、国家自然基金、公益性行业（农业）专项、黑龙江省科学基金、哈尔滨市人才创新项目、中国博士后科学基金等40余项课题，发表SCI收录文章100余篇，出版专著11部，获得省部级奖10项，获得发明专利20项。目前，团队与美国堪萨斯州立大学、德国柏林自由大学、英国帝国理工大学及清华大学等建立了良好的合作关系。

团队现有固定研究人员11人，其中研究员2人、副研究员5人、助理研究员4人。在读硕士/博士研究生20余人。

研究方向包括：

1. 动物结核病研究

以结核分枝杆菌、牛分枝杆菌和副结核分枝杆菌等为对象，开展流行病学、转录调控及致病机制研究，研发新型的防控制剂。

2. 病原菌耐药机制研究

以动物重要病原菌为对象，开展细菌耐药流行病学，阐明耐药形成及传播机制，研发安全、高效的抗菌制剂。

3. 猪呼吸系统疾病研究

开展猪呼吸道细菌病（副猪嗜血杆菌和胸膜肺炎放线杆菌）的病原生物学、流行病学、致病机理及基因工程疫苗研究。

4. 食源性细菌病研究

以沙门氏菌和单增李斯特菌等重要食源性致病菌为对象，开展流行规律、风险评估及防控策略研究；研发新型防控制剂。

5. 动物链球菌病研究

开展动物链球菌流行病学、毒力因子及功能、复制机制、致病机理和防控技术研究。

1、动物结核病等重要细菌病的流行病学研究

      开展动物结核病、牛副结核病的流行病学研究，阐明流行特点、分布及流行规律，制定相应的防控策略和方案。

2、牛分枝杆菌等重要病原菌持续感染和致病机理研究

     开展牛分枝杆菌等重要动物病原菌与宿主的相互研究，揭示重要病原菌持续感染和致病机理。

3、动物结核病等重要细菌病新型防控制剂研究

      研发针对动物结核病等的安全、高效防控制剂，包括新型疫苗、诊断技术和抗菌制剂。

一、发表SCI文章（2017-2021）

2017年SCI文章

1       ifangLiu, Liping Chen, Wei S, Chunlai Wang, Huanan Wang, Fangna Zhu, Guangxing Li*, Siguo Liu*. Physiology and pathogenicity of cpdB deletedmutant of avian pathogenic Escherichia coli. Research in Veterinary Science. 2017,111:21-25.

2       ngXie, Gang Li, Yanhe Zhang, Long Zhou, Shuanghong Liu, Siguo Liu, Chunlai Wang. Attenuated Actinobacillus pleuropneumoniaedouble-deletion mutant ∆clpP/apxIIC confers protection against homologous orheterologous strain challenge. BMC Veterinary Research. 2017, 13(14):1-10.

3       Yanhe Zhang, Gang Li, Fang Xie, Siguo Liu, Chunlai Wang. Evaluation of glutathione-binding protein Aof Haemophilus parasuis as a vaccine candidate in a mouse model. Journal of Veterinary Medical Science. 2017, 79(1):184-187.

4       Fuguang Chen, Fang Xie,Baoling Yang, Chengcheng Wang, Siguo Liu*, and Yueling Zhang*. Streptococcussuis sortase A is Ca 2+independent and is inhibited by acteoside,isoquercitrin and baicalin. pLoS One. 2017, 12(3):e0173767.

5       Wei Si, Shenye Yu, Henggui Liu, Chunlai Wang,Liping Chen, Gaoling Wang, Jianan Wu, Siguo Liu*. Bacterial ghostimproving the immunological efficacy of Newcastle disease inactivated vaccine. Veterinary Microbiology. 2017, 203:189-195.

6       FangXie, Gang Li, Yanhe Zhang, Long Zhou, Shuanghong Liu, Siguo Liu, Chunlai Wang. Pyridoxal phosphate synthases PdxS/PdxT arerequired for Actinobacillus pleuropneumoniae viability, stress tolerance andvirulence. pLoSOne. 2017,12(4):e0176374.

7       FangXie, Yalei Wang, Gang Li, Shuanghong Liu, Ning Cui, Siguo Liu, Paul R Langford,Chunlai Wang. The SapA transproter of Actinobacillus pleuropneumoniae confersresistance to antimicrobial peptide PR-39. Frontiers in Microbiology. 2017, 8: 811.

8       GangLi, HuiNiu, Yanhe Zhang, Fang Xie,Ting Li, Shengkui Xu, Siguo Liu, ChunlaiWang*. Haemophilus parasuis Cytolethal Distending Toxin Induces Cell CycleArrest and p53-dependent Apoptosis. PLoS One. 2017, 12(5): e0177199.

9       GangLi, Fang Xie, Jianjun Li, Yanhe Zhang, Ting Li, Shengkui Xu, Paul R. Langford,Siguo Liu, Chunlai Wang*. Identification of novel vaccine candidates ofHaemophilus parasuis serovar 5 by immunoproteomics. Journal of Proteomics. 2017, 163:111-117.

10    Jun Cao, Fei Yi, Qiufeng Tian, Guanghui Dang,Tongli Zhang, Manyu Wang, Siguo Liu*,Shenye Yu*. Targeting the gram-negative bacteria peptidoglycan synthase MraY asa new approach for monoclonal antibody therapy. Human Vaccines & Immunotherapeutics. 2017,13(9):2086-2091.

11   Xiu-MeiWang, Zhimin Dong, Stefan Schwarz, Zhu Yao, Xin Hua, Yanhe Zhang, Siguo Liu* and Wan-Jiang Zhang*. Plasmids of diverse Incgroups mediate the dissemination of the fosfomycin resistance gene fosA3 inEscherichia coli from pigs, chickens and dairy cows in Northeast China. Antimicrobial Agents andChemotherapy. 2017 61(9):e00859.

2018年SCI文章

12    Tian Qiu-feng*, Zhou Wei*, Si Wei, Yi Fei, HuaXin, Yue Min, Chen Li-ping, Liu Si-guo, Yu Shen-ye. Construction of SalmonellaPullorum ghost by co-expression of lysis gene E and the antimicrobialpeptide SMAP29 and evaluation of its immune efficacy in specific-pathogen-freechicks. Journalof Integrative Agriculture. 2018, 17(1): 197–209.

13    Fangyan Yuan,Jinlin Liu, Wujin You, Weicheng Bei, Chunlai Wang, Jin Zhao, Yiongxiang Tian, Siguo Liu*, Generation, safety and immunogenicity of an Actinobacillus pleuropneumoniaequintuple deletion mutant SLW07 (ΔapxICΔapxIICΔorf1ΔcpxARΔarcA), Vaccine. 2018, 36(14):1830-1836.

14    Xiumei Wang,Yao Zhu, Xin Hua, Fuguang Chen, Changzhen Wang, Yanhe Zhang, Siguo Liu*, Wanjiang Zhang*, F14: A-: B- and IncX4 Inc group cfr-positiveplasmids circulating in Escherichia coli of animal origin in NortheastChina. Veterinary Microbiology. 2018, 217: 53–57.

15    Guanghui Dang,Yingying Cui, Lei Wang, Tiantian Li, Ziyin Cui, Ningning Song, Liping Chen, Siguo Liu*, Extracellular Sphingomyelinase Rv0888 of Mycobacteriumtuberculosis Contributes to Pathological Lung Injury of M. smegmatisin Mice via Inducing Formation of Neutrophil Extracellular Traps. Frontiers in Immunology.2018, 9:677.

16    Xin Hua, Qin Yang,Wanjiang Zhang, Shenye Yu, Zhimin Dong, and Stefan Schwarz, Siguo Liu*. Antibacterialactivity and mechanism of action of aspidinol against multi-drug resistantmethicillin-resistant Staphylococcusaureus. Frontiers in Pharmacology, 2018, 9: 619.

17   Pengpeng Xia, Guomei Quan, Yi Yang, Jing Zhao, YitingWang, Mingxu Zhou,Philip R. Hardwidge, Jianzhong Zhu, Siguo Liu and Guoqiang Zhu, Binding determinants in theinterplay between porcine aminopeptidase N and enterotoxigenic Escherichia coli F4 fimbriae, VeterinaryResearch. 2018, 49:23.

2019年SCI文章

18   Zhe Li, Hongxiu Liu, Guanghui Dang, Ziyin Cui,Ningning Song, Quankai Wang, Siguo Liu*, Liping Chen*. PE17 Protein of Mycobacteriumtuberculosis Enhances Mycobacterium smegmatis Survival inMacrophages and Pathogenicity in Mice. Microbial pathogenesis. 2019, 126:63–73.

19    Fuguang Chen#,Wanjiang Zhang#, Stefan Schwarz, Yao Zhu, Ruichao Li, Xin Hua, Siguo Liu*.Genetic characterization of a multidrug resistance/virulence genomic elementcarrying two T6SS gene clusters in a clinical Klebsiella pneumoniae isolate of swine origin. Journalof Antimicrobial Chemotherapy. 2019 Jun 1;74(6):1539-1544.

20    Xue Bai, Siguo Liu, JianjunZhao, Yuening Cheng, Hailing Zhang, Bo Hu, Lei Zhang, Qiumei Shi, ZhiqiangZhang, Tonglei Wu, Guoliang Luo, Shizhen Lian, Shujuan Xu, Jianke Wang,Wanjiang Zhang, Xijun Yan. Epidemiology and molecular characterization of theantimicrobial resistance of Pseudomonasaeruginosa in Chinese mink infected by hemorrhagic pneumonia. Canadian Journal of Veterinary Research. 2019Apr;83(2):122-132.

21    Yongjie Jia, Wei Si, Zhimin Hong, Mingren Qu, Nianhua Zhu, Siguo Liu* and Guanhong Li*,Toll-like receptor 2-mediated induction of avian β-defensin 9 by Lactobacillus rhamnosus and its cellularcomponents in chicken intestinal epithelial cells. Food and Agricultural Immunology. 2019; 30 (1): 398-417.

22   Ningning Song, Zhaoli Li, Ziyin Cui, Liping Chen,YingyingCui, Guanghui Dang, Zhe Li, He Li, Siguo Liu*.The prominent alteration in transcriptome and metabolome of Mycobacteriumbovis BCG str. Tokyo 172 induced by vitamin B1. BMC Microbiology. 2019,19,104.

23    Yao Zhu#, Wanjiang Zhang#, Stefan Schwarz, Changzhen Wang,Wenyu Liu, Fuguang Chen, Tian Luan and SiguoLiu*. Characterization of ablaIMP-4-carrying plasmid from Enterobactercloacae of swine origin. Journal of AntimicrobialChemotherapy. 2019 Mar 16.pii:dkz107.doi:10.1093/jac/dkz107.

24   Wanjiang Zhang#, Yao Zhu#, Changzhen Wang, Wenyu Liu,Ruichao Li, Fuguang Chen, Tian Luan, Stefan Schwarz, and Siguo Liu*. Characterizationof multidrug-resistant porcine Klebsiella pneumonia ST11 strain coharbouringbla KPC-2 and fosA3 on two different hybrid plasmids. mSphere. 2019,4(5): e00590-19.

25   Xin Hua, Qin Yang, Wanjiang Zhang, Yue Jia, Zhimin Dongand SiguoLiu*. Transcriptional Analysis of the Effects of Gambogic acid and Neogambogicacid: Inhibition of Staphylococcus aureusand methicillin-resistant Staphylococcusaureus. Frontiers in Pharmacology.2019, 10: 986.

26   LifengXiong, Elaine Chan, Jade L. L. Teng, Siguo Liu, Susanna K. P. Lau,* andPatrick C.Y.Woo,* Malate-Dependent Carbon Utilization Enhances CentralMetabolism and Contributes to Biological Fitness of Laribacter hongkongensisvia CRP Regulation, Front Microbiol. 2019; 10: 1991.

27   He Li#, Guanghui Dang#, Ziyin Cui, Ningning Song, Liping Chen, Siguo Liu*, Identification of a novel Mycobacterium tuberculosis serineprotease Rv3194c on activity and pathogenicity. Tuberculosis, 2019,119:101880.

28   Fang Xie#, Yanan Zan#, Yueling Zhang, Ning Zheng,Qiulong Yan, Wanjiang Zhang, Fuguang Chen, Xinyuan Zhang, Siguo Liu*, Streptococcussuis cysteine proteaseApdS promotes evasion of innate immune defenses by cleaving the antimicrobialpeptide cathelicidin LL-37. Journal of Biological Chemistry, 2019 Nov 22; 294(47):17962-17977.

29   Chunlai Wang#, Peng Li#, Jian Sun#, QiulongYan, Liping Chen, Bo Dong, Hongbo Ni, Xiaoxuan Zhang, Tiantian Li, WanjiangZhang, He Li, Xiuyan Han, Siyi zhang, Miao Xu, Yufang Ma, Shenghui Li*,Siguo Liu*. Characterization of the pig gut microbiome and antibioticresistome in industrialized feedlots in China. mSystems. 2019, 4(6): e00206-19.

2020年SCI文章

30   Hongxiu Liu#, guanghui dang#, XinxinZang, Zhuming Cai, Ziyin Cui, Ningning Song, Siguo Liu*. Characterization andpathogenicity of extracellular serine protease MAP3292c from Mycobacterium avium subsp.paratuberculosis, Microbial pathogenesis. 2020, 142, 104055.

31    Fang Xie, Yanan Zan, Xinyuan Zhang, Huihui Zhang, Mingjie Jin, Wanjiang Zhang,Yueling Zhang, Siguo Liu*. DifferentialAbilities of Mammalian Cathelicidins to Inhibit Bacterial Biofilm Formation andPromote Multifaceted Immune Functions of Neutrophils, Internationaljournal of molecular sciences, 2020, 21(5), 1871.

32   Gang Li, Qian Zhao, Tian Luan, Yangbo Hu, Yueling Zhang,Ting Li, Chunlai Wang, Fang Xie, Paul Langford, and Siguo Liu*. BasalLevel Effects of (p)ppGpp in the Absence of Branched Chain Amino Acids in Actinobacillus pleuropneumoniae, Journal of Bacteriology. 2020, 202 (8):e00640-19.

33   Ningning Song, Yongqiang Zhu,Yingying Cui, Mingyue LV, YiyiTang, Ziyin Cui, Guanghui Dang, Huajun Zheng* and Siguo Liu*. Vitamin Band Vitamin C affect DNA methylation and amino acid metabolism in Mycobacterium bovis BCG, Frontiers in Microbiology. 2020,11:812.

34   Zhu Yao, Liu Wenyu, Schwarz Stefan, Wang Changzhen,Yang Qin, Luan Tian, Wang Lingli, Liu Siguo, Zhang Wanjiang*. Characterizationof a blaNDM-1- carrying IncHI5 plasmid from Enterobacter cloacae of food animalorigin, Journal of Antimicrobial Chemotherapy. 2020,75(5):1140-1145.

35   Ping Chen, Ran Liu, Mengmeng Huang, Jinlu Zhu,Dong Wei, Francis J. Castellino, Guanghui Dang, Fang Xie, Gang Li, Ziyin Cui, Siguo Liu*, Yueling Zhang*. A unique combination of glycoside hydrolases in Streptococcus suis specifically andsequentially acts on host-derived αGal-epitope glycans, Journal of Biological Chemistry. 2020 Jul 31;295(31):10638-10652.

36   YaoZhu, Wanjiang Zhang, Changzhen Wang, Wenyu Liu, Qin Yang, Tian Luan, LingliWang, Stefan Schwarz and Siguo Liu*, Dentification of the noveloptrA-harbouring transposon Tn6823 in Staphylococcusaureus of chicken origin, Journal of Antimicrobial Chemotherapy. 2020,75(5):1140-1145.

37   Ziyin Cui, Guanghui Dang, Ningning Song, Yingying Cui,Zhe Li, Xinxin Zang, Hongxiu Liu, Zhongxing Wang, Mingyue Lv, Zhuming Cai, YiyiTang, Siguo Liu*. Rv3091, an extracellular patatin-like phospholipase Aof Mycobacterium tuberculosis,enhanced intracellular survival of bacteria by macrophage phagosome escape, Frontiers in Microbiology. 2020,11:2204.

38   Yao Zhu, Lei Liu, Stefan Schwarz, Wenyu Liu,Changzhen Wang, Qin Yang, Tian Luan, Lingli Wang, Siguo Liu, andWanjiang Zhang*.Characterization of a novel hybridplasmid coharboring blaKPC-2 and qnrVC4 in a clinical Citrobacter freundiistrain, AntimicrobialAgents and Chemotherapy, 2020Oct 20;64(11):e01379-20.

39    Duan Q, Pang S, Wu W, Jiang B, Zhang W, LiuS, Wang X, Pan Z, Zhu G. A multivalent vaccine candidate targetingenterotoxigenic Escherichia coli fimbriae for broadly protecting againstporcine post-weaning diarrhea. VetRes. 2020 Jul 23;51(1):93.

2021年SCI文章

40    Zhu Yao, Wang Changzhen, Schwarz Stefan, LiuWenyu, Yang Qin, Luan Tian, Wang Lingli, Liu Siguo, Zhang Wanjiang*, Identificationof a novel tetracycline resistance gene, tet(63), located on a multiresistanceplasmid from Staphylococcus aureus, Journal of Antimicrobial Chemotherapy. 2021 Mar 12;76(4): 1102-1104.

41    Zhu, Yao; Zhang, Wanjiang; Liu, Siguo;Schwarz, Stefan Identification of an IS431-derived translocatable unitcontaining the erm(C) gene in Staphylococcus aureus, Journal of AntimicrobialChemotherapy. 2021 Feb 11;76(3):576-581.

42    Binjie Chen, Xianchen Meng, Jie Ni, MengpingHe, Yanfei Chen, Pengpeng Xia, Heng Wang, Siguo Liu, Guoqiang Zhu, XiaMeng. Positiveregulation of Type III secretion effectors and virulence by RyhB paralogs inSalmonella enterica serovar Enteritidis. Vet Res. 2021Mar 10;52(1):44.

43    Yue Jia, Qing Yang, Wanjiang Zhang, Gang Li, Siguo Liu* andXin Hua*, Antibacterial Activity and Proteomics Studies of Berberine Against Haemophilus parasuis, Research in Veterinary Science.2021, Jun 5;138: 62-68.

44    Stefan Schwarz, Wanjiang Zhang, Xiang-Dang Du,Henrike Krüger, Andrea T Feßler, Shizhen Ma, Yao Zhu, Congming Wu, JianzhongShen, Yang Wang. Mobile Oxazolidinone Resistance Genes in Gram-Positive andGram-Negative Bacteria, Clin Microbiol Rev. 2021 Jun 2;e0018820.

45    Qin Yang, YaoZhu, Schwarz Stefan, Lingli Wang, Wenyu Liu, Wenlin Yang, Tian Luan, Siguo Liu,and Wanjiang Zhang. A novel plasmid from Aerococcus urinaeequi of porcineorigin co-harboring the tetracycline resistance genes tet(58) and tet(61), Veterinary Microbiology. 2021, 257, 109065.

二、获奖成果

1.       人兽共患性结核病新型诊断技术及其在牛结核控制和净化中的应用，湖北省技术发明二等奖，排名第3，2020年度

2.       人畜间结核病流行病学的研究及预警模型的建立和应用，新疆自治区科技进步三等奖，（李爱巧，刘思国，刘剡，呼西丹·阿巴拜克力，杨启元，王文秀，王六合），2017年度；

3.       人畜间结核病流行病学的调查研究及推广应用，农业部2014-2016年全国农牧渔业丰收奖成果三等奖（李爱巧，呼西旦·阿巴拜克力，刘思国，杨启元，施远翔，王六合，陈利苹，李君莲，陈飞，帕提玛·努合曼，程维疆，何生，郭亚军，王爱菊，方新元，张成成，郭新钢，刘军成，周棕长，王杏芹，赛力克江，韩义忠，舒峰，彭华刚，罗文毅），2016年；

4.       牛结核病病原生态学和流行病学研究，黑龙江省政府，黑龙江省自然科学三等奖（刘思国，司微，于申业，王牟平，陈利苹），2014年；

5.       牛结核病病原生态学和流行病学研究，中国农业科学院科学技术成果奖，二等奖（刘思国，刘慧芳，司微，王春来，于申业，王牟平），2012年；

6.       奶牛结核病间接ELISA检测方法的研究，哈尔滨市科技进步三等奖（刘思国，郭设平，王春来，王牟平），2006年；

7.       奶牛结核病快速诊断技术研究，哈尔滨市科技进步三等奖（刘思国，郭设平，王春来，王牟平），2005年；

8.         奶牛结核病快速诊断技术研究，中国农业科学院科学技术成果三等奖（刘思国，郭设平，王春来，王牟平），2005年。

三、专利