项目围绕两个方向：

（1） 针对猪链球菌及其它相关链球菌，发现参与肽聚糖合成和细胞分裂这一基础代谢过程的重要蛋白，解析其功能和作用机制，为开发新型链球菌及细菌抗菌药物靶标奠定理论基础。

（2）采用猪链球菌表面展示的方法，开发猪链球菌病及重要动物病毒病的多联疫苗，为动物病防治提供新材料、新思路。

1.  国家自然科学基金面上项目：猪链球菌A型PBPs在胞壁肽聚糖合成中的功能和分工及其机制研究（32172853，2022.01 -2025.12），58万。

2.  国家自然科学基金面上项目：猪链球菌的eSTK/eSTP系统对反应调节因子CovR的调控及其机制研究（31772757，2018.01 -2021.12），62万。

3.  “十三五”国家重点研发计划：“畜禽重要病原菌的病原组学与网络调控研究”项目（2017YFD0500200）子课题“病原菌毒力调控机制研究”（2017YFD0500203，2017.07-2021.06），105万。

4.  黑龙江省科学基金面上项目：2型猪链球菌SSU05_0196蛋白结合纤溶酶原及介导血行播散的分子机制（C2017075，2017.10-2020.10），6万，结题。

5.  国家自然科学基金青年项目：SK2b型A族链球菌激活纤溶酶原的分子机制的研究（31500112，2016.01-2018.12），19万。

1.       Ping Chen, Ran Liu, Mengmeng Huang, Jinlu Zhu, Dong Wei, Francis J. Castellino,Guanghui Dang, Fang Xie, Gang Li, Ziyin Cui, Siguo Liu*, and Yueling Zhang*. A unique combination of glycosidehydrolases in Streptococcus suis specifically and sequentially acts onhost-derived alpha Gal-epitope glycans. Journalof Biological Chemistry, 2020, 295(31): 10638-10652.

2.       Fuguang Chen, Fang Xie, Baoling Yang,Chengcheng Wang, Siguo Liu*, and YuelingZhang*, Activity of Sortase A from Streptococcus suis is Ca2+-Independentand Strongly Inhibited by Aceteoside, Isoquercitrin and Baicalin, PlosOne, 2017, 12(3):e0173767.

3.       Yueling Zhang, Jeffrey Mayfield, Victoria A. Ploplis, and Francis J. Castellino. Theβ-domain of cluster 2b streptokinase is a major determinant for the regulationof its plasminogen activation activity bycellular plasminogen receptors. Biochemicaland Biophysical Research Communications, 2014, 444(4):595-8.

4.       Yueling Zhang, Zhong Liang, Kristofor Glinton, Victoria A. Ploplis, Francis J.Castellino. Functional differences between Streptococcuspyogenes cluster 1 and cluster 2b streptokinases are determined by their β-domains. FEBS Letters. 2013, 587(9):1304-9.

5.       Zhong Liang*, Yueling Zhang*, Garima Agrahari, Vishwanatha Chandrahas, KristoforGlinton, Deborah L. Donahue, Rashna D. Balsara, Victoria A. Ploplis, andFrancis J. Castellino. A natural inactivating mutation in the CovS component of the CovRS regulatory operonin a pattern D Streptococcal pyogenesstrain influences virulence-associated genes. Journal of Biological Chemistry. 2013, 288(9):6561-73.

6.       Yueling Zhang, Zhong Liang, Hsing-Tse Hsueh,Victoria A. Ploplis, and Francis J. Castellino. Characterization of streptokinases from group A streptococcireveals a strong functional relationship that supports the coinheritance ofplasminogen-binding M-protein and cluster 2B streptokinase. Journal of Biological Chemistry.2012, 287(50): 42093-103.

7.       Yueling Zhang, Jiansong Ju, Hao Peng, Feng Gao, Cheng Zhou, Yan Zeng, Yanfen Xue, Yin Li, Bernard Henrissat, George F. Gao, Yanhe Ma. Biochemicaland structural characterization of the intracellular mannanase AaManA reveals anovel glycoside hydrolase family belonging to GH-A superfamily. Journalof Biological Chemistry. 2008, 283(46): 31551-58.

8.       Yueling Zhang, Feng Gao, Yanfen Xue, Yan Zeng, Hao Peng, Jianxun Qi and Yanhe Ma.Crystallization and preliminary X-ray study of a mannanase from Alicyclobacillus acidocaldarius Tc-12-31.Acta Crystallographica Section F,Structural Biology and CrystallizationCommunications. 2008, 64(3): 209-12.

9.       Cheng Yi, Guangyue Li, Wenmeng Wang, Yixuan Sun, Yueling Zhang, Chen Zhong, Daniel B. Stovall, Dangdang Li, JinmingShi, and Guangchao Sui. 2021. Disruption of YY1-EZH2 interaction using syntheticpeptides inhibits breast cancer development. Cancers. 2021; 13(10):2402.

10.    Fang Xie, Yanan Zan, Xinyuan Zhang, Huihui Zhang, Mingjie Jin, WanjiangZhang, Yueling Zhang, and Siguo Liu.Differential abilities of mammalian cathelicidins to inhibit bacterial biofilmformation and promote multifaceted immune functions of neutrophils. InternationalJournal of Molecular Sciences. 2020, 21:1871.

11.    Gang Li, Qian Zhao, Tian Luan, Yangbo Hu, Yueling Zhang, Ting Li, Chunlai Wang, FangXie, Wanjiang Zhang, Paul R.Langford, and Siguo Liu. Basal-level effects of(p)ppGpp in the absence of branched-chain amino acids in actinobacilluspleuropneumoniae. Journal of Baceriology 2020, 202(8): e00640-19.

12.    Fang Xie, Yanan Zan, Yueling Zhang,Ning Zheng, Qiulong Yan, Wanjiang Zhang, Huihui Zhang, Mingjie Jin, FuguangChen, Xinyuan Zhang, and Siguo Liu. (2019) The cysteine protease ApdS from Streptococcussuis promotes evasion of innate immune defenses by cleaving theantimicrobial peptide cathelicidin LL-37. Journalof Biological Chemistry, 294(47): 17962-17977.

13.    Martina LSanderson-Smith, Yueling Zhang,Diane Ly, Deborah Donahue, Andrew Hollands, Victor Nizet, Victoria Ploplis,Mark Walker, Francis Castellino. A key role for the urokinaseplasminogen activator (uPA) in invasive Group A streptococcal infection. PLOS Pathogen, 9(7): e1003469.doi:10.1371/journal.ppat.1003469.

14.    YingyingZheng, Yanfen Xue, Yueling Zhang, Cheng Zhou, Ulrich Schwaneberg, Yanhe Ma. Cloning,expression, and characterization of a thermostable glucoamylase from Thermoanaerobactertengcongensis MB4. AppliedMicrobiology and Biotechnology, 2010, 87(1): 225-33.

15.    Cheng Zhou,Yanfen Xue, Yueling Zhang, Yan Zeng,and Yanhe Ma. Recombinant Expression and characterization of Thermoanaerobacter tengcongensisthermostable α-glucosidase with regioselectivity for high-yieldisomaltooligosaccharides synthesis. Journal of Microbiology and Biotechnology.2009, 19(12): 1547–56.

16.    杨宝玲，陈福广，谢芳，刘思国，沈国顺*，张跃灵*。猪链球菌 2 型SSU05-1311 蛋白的表达纯化和多克隆抗体的制备。中国兽医科学，2016，46(03)：310-314。

17.    杨宝玲，陈福广，谢芳，刘思国，沈国顺*，张跃灵*。2 型猪链球菌SsU05-0474 的表达纯化及单克隆抗体制备。中国动物传染病学报，2016，24(2)：25-30。

18.    刘冉，黄萌萌，陈平，朱金鲁，谢芳，刘思国*，张跃灵*。猪链球菌2型05ZYH33菌株启动子的筛选和鉴定。中国预防兽医学报，2019，41(06)：584-589。

19.    陈平，刘冉，黄萌萌，朱金鲁，谢芳，倪宏波，刘思国*，张跃灵*。猪链球菌2型05ZYH33菌株具有N-乙酰氨基葡萄糖苷酶活性蛋白的鉴定及功能研究。中国预防兽医学报，2019，41(05)：462-467。

20.    刘冉，陈福广，陈平，黄萌萌，朱金鲁，谢芳，孟庆文，刘思国，刘建华*，张跃灵*。利用信息肽诱导和Cre/LoxP系统构建猪链球菌基因缺失株。中国预防兽医学报，2019，41(02)：125-130。

21.    黄萌萌，刘冉，陈平，朱金鲁，卫东，谢芳，李刚，刘思国，张跃灵*。猪链球菌表面蛋白展示系统的构建及展示蛋白的初步观察。中国预防兽医学报，2020，42(02)：105-113。

22.    朱金鲁，陈平，黄萌萌，刘冉，卫东，刘思国，张跃灵*。提高表达分选酶A的猪链球菌突变株的构建及初步评价[J]。中国预防兽医学报，2021，43(01)：7-13.