“茶树营养与养分管理技术团队”于2013年成立，团队现有固定成员11人，其中研究员3名，副研究员6名，国家茶叶产业技术体系岗位专家2名。开展茶树养分吸收利用与品质成分代谢生物学机制、茶园土壤氮碳转化循环特性与关键影响因子解析、茶园养分综合管理技术创新与应用三方面的研究。“十三五”期间主持各类科技项目24项，其中主持国家“十三五”重点研发计划项目1项和课题1项、国家自然科学基金5项、国际合作项目2项、浙江省自然科学基金项目3项，承担国家茶叶产业技术体系岗位2个，累计合同经费7595万元、留所合同经费2945万元。在关键营养元素对茶树生长与品质成分代谢中的影响机制、非生物因子调控茶叶品质成分代谢的作用机制、长期施肥对茶园土壤质量、微生物群落和碳氮转化的影响机制，以及我国茶区化肥减施增效技术研究等方面取得重要进展。提出了茶类养分投入定额、养分基准配方、有机肥替代与运筹等关键技术，形成了以茶树营养需求、茶类品质特征和生态环境约束的多目标茶树养分管理技术体系，解决了“施啥肥、施多少、何时施、怎么施、安全施”等问题。针对我国茶叶生产小农户多、高度分散、测土配方施肥难覆盖等问题，瞄准区域茶类、土壤和气候等特点，将多个关键技术进行集成、简化，形成易推广、简操作、高适应的主要产茶区茶园化肥减施技术模式，将施肥服务对象从田块尺度扩展到区域尺度，提高了技术覆盖率，取得了显著的经济、社会和生态效益。“十三五”期间，团队共发表学术论文78篇，其中SCI期刊论文39篇（JCR排名TOP 5%期刊论文5篇）；主编著作2部，参编著作3部；申报专利31件（发明专利14件），获得专利授权26件（发明专利10件）；获软件著作权11项；发布农业农村部主推技术3项、施肥技术指南6项；参加获得中华农业科技奖二等奖一项（排名第4）、三等奖一项（排名第9）。依托研究所和团队建有国家农业科学土壤质量西湖观测实验站，管理运行的茶园化肥氮用量、养分综合管理技术、水肥一体化、茶树品种与养分耦合等长期定位试验，参与国家土壤质量数据中心，承担部省级茶园土壤质量长期监测任务。团队配备元素分析仪、ICP-OES、ICP-MS、温室气体分析仪、液相色谱仪等设备，试验与分析条件完善。

2018-2020年发表论文：

1.        Yang, X., Ni, K., Shi, Y., Yi, X., Ji, L., Ma, L., Ruan, J. (2020):Heavy nitrogen application increases soil nitrification throughammonia-oxidizing bacteria rather than archaea in acidic tea (Camellia sinensisL.) plantation soil. Science of The Total Environment 717, 137248.

2.        Liu, MY, Tang, DD., Zhang QF., Ruan, J. (2020) iTRAQ-based proteomicanalysis provides insights into the biological mechanism of ammonium metabolismin tea plant (Camellia sinensis L.). Acta Physiologiae Plantarum DOI: https://doi.org/10.1007/s11738-020-03037-9

3.        Zhang, Q., Hu, J., Liu, M., Shi, Y., De Vos, R. C. H., Ruan, J.(2020): Stimulated biosynthesis of delphinidin-related anthocyanins in teashoots reducing the quality of green tea in summer. Journal of the Science ofFood and Agriculture. DOI: 10.1002/jsfa.10158

4.        Gao, Y., Xu, Y. Q., Ruan, J., Yin, J. F. (2020): Selenium affectsthe activity of black tea in preventing metabolic syndrome in high-fat diet-fedSprague-Dawley rats. Journal of the Science of Food and Agriculture 100(1),225-234.

5.        Ji, L., Ni, K., Wu, Z., Zhang, J., Yi, X., Yang, X., Ling, N., You,Z., Guo, S., Ruan, J. (2020): Effect of organic substitution rates on soilquality and fungal community composition in a tea plantation with long-termfertilization. Biology and Fertility of Soils. Doi: 10.1007/s00374-020-01439-y

6.        Guo, J., Ling, N., Chen, Z., Xue, C., Li, L., Liu, L., Gao, L.,Wang, M., Ruan, J., Guo, S., Vandenkoornhuyse, P., Shen, Q. (2020): Soil fungalassemblage complexity is dependent on soil fertility and dominated bydeterministic processes. New Phytologist doi:10.1111/nph.16345.

7.        Fan, K., Zhang, Q., Tang, D., Shi, Y., Ma, L., Liu, M., Ruan, J.(2020): Dynamics of nitrogen translocation from mature leaves to new shoots andrelated gene expression during spring shoots development in tea plants(Camellia sinensis L.). Journal of Plant Nutrition and Soil Science 183(2),180-191.

8.        Tang, D. D., Liu, M. Y., Zhang, Q. F., Ma, L. F., Shi, Y. Z., Ruan,J. Y. (2020): Preferential assimilation of NH4+ over NO3- in tea plantassociated with genes involved in nitrogen transportation, utilization andcatechins biosynthesis. Plant Science 291. doi:10.1016/j.plantsci.2019.110369.

9.        Liu, M. Y., Tang, D. D., Shi, Y. Z., Ma, L. F., Li, Y., Zhang, Q.F., Ruan, J. Y. (2019): Short-term inhibition of glutamine synthetase leads toreprogramming of amino acid and lipid metabolism in roots and leaves of teaplant (Camellia sinensis L.). Bmc Plant Biology 19(1), 425. 

10.    Yang, X. D., Ma, L. F., Ji, L. F., Shi, Y. Z., Yi, X. Y., Yang, Q.L., Ni, K., Ruan, J. Y. (2019): Long-term nitrogen fertilization indirectlyaffects soil fungi community structure by changing soil and pruned litter in asubtropical tea (Camellia sinensis L.) plantation in China. Plant and Soil444(1-2), 409-426. 

11.    Ma, L. F., Shi, Y. Z., Ruan, J. Y. (2019): Nitrogen absorption by field-growntea plants (Camellia sinensis) in winter dormancy and utilization in springshoots. Plant and Soil 442(1-2), 127-140. 

12.    Zhang, Q., Wu, S., Li, Y., Liu, M., Ni, K., Yi, X., Shi, Y., Ma, L.,Willmitzer, L., Ruan, J. (2019): Characterization of three different classes ofnon-fermented teas using untargeted metabolomics. Food Research International121, 697-704. 

13.    Dong, F., Hu, J., Shi, Y., Liu, M., Zhang, Q., Ruan, J. (2019):Effects of nitrogen supply on flavonol glycoside biosynthesis and accumulationin tea leaves (Camellia sinensis). Plant Physiology and Biochemistry 138,48-57. 

14.    Fan, K., Zhang, Q., Liu, M., Ma, L., Shi, Y., Ruan, J. (2019):Metabolomic and transcriptional analyses reveal the mechanism of C, Nallocation from source leaf to flower in tea plant (Camellia sinensis. L).Journal of Plant Physiology 232, 200-208. 

15.    Zhang, Q., Tang, D., Liu, M., Ruan, J. (2018): Integrated analysesof the transcriptome and metabolome of the leaves of albino tea cultivarsreveal coordinated regulation of the carbon and nitrogen metabolism. ScientiaHorticulturae 231, 272-281. 

16.    Yang, X. D., Ni, K., Shi, Y. Z., Yi, X. Y., Zhang, Q. F., Fang, L.,Ma, L. F., Ruan, J. Y. (2018): Effects of long-term nitrogen application onsoil acidification and solution chemistry of a tea plantation in China.Agriculture Ecosystems & Environment 252, 74-82.

17.    Ji, L., Wu, Z., You, Z., Yi, X., Ni, K., Guo, S., Ruan, J. (2018):Effects of organic substitution for synthetic N fertilizer on soil bacterial diversityand community composition: A 10-year field trial in a tea plantation.Agriculture, Ecosystems & Environment 268, 124-132. 

18.    Dong, F., Shi, Y., Liu, M., Fan, K., Zhang, Q., Ruan, J. (2018):iTRAQ-based quantitative proteomics analysis reveals the mechanism underlyingthe weakening of carbon metabolism in chlorotic tea leaves. InternationalJournal of Molecular Sciences 19(12), 3943. 

19.    KC, S., Liu, M., Zhang, Q., Fan, K., Shi, Y., Ruan, J. (2018):Metabolic changes of amino acids and flavonoids in tea plants in response toinorganic phosphate limitation. International Journal of Molecular Sciences19(11), 3683. 

20.    Yang, Y. Y., Wang, F., Wan, Q., Ruan, J. Y. (2018): Transcriptomeanalysis using RNA-Seq revealed the effects of nitrogen form on major secondarymetabolite biosynthesis in tea (Camellia sinensis) plants. Acta PhysiologiaePlantarum 40(7). doi:10.1007/s11738-018-2701-0. 

21.    Ni, K., Wang, J., Zhang, Q., Yi, X., Ma, L., Shi, Y., Ruan, J.(2018): Multi-element composition and isotopic signatures for the geographicalorigin discrimination of green tea in China: A case study of Xihu Longjing.Journal of Food Composition and Analysis 67, 104-109. 

22.    阮建云, 马立锋, 伊晓云, 石元值, 倪康, 刘美雅, 张群锋 (2020): 茶树养分综合管理与减肥增效技术研究. 茶叶科学40(01), 85-95.

23.    杨清霖, 杨向德, 季凌飞, 马立锋, 阮建云 (2020): 滴灌施肥对幼龄茶树生长和养分吸收的影响. 茶叶科学40(01), 96-104.

24.    倪康, 廖万有, 伊晓云, 牛司耘, 马立锋, 石元值, 张群峰, 刘美雅, 阮建云 (2019): 我国茶园施肥现状与减施潜力分析. 植物营养与肥料学报25(03), 421-432.

25.    汤丹丹, 刘美雅, 张群峰, 石元值, 马立锋, 阮建云 (2019): 不同氮素形态、pH对茶树元素吸收及有机酸含量影响. 茶叶科学39(02), 159-170.

26.    牛司耘, 倪康, 赵晨光, 马立锋, 阮建云 (2019): 不同地区茶园土壤硝化潜势特征研究. 茶叶科学39(06), 731-741.

27.     汤丹丹，刘美雅，张群峰等.茶树胞质型谷氨酰胺合成酶基因CsGS1s的克隆及表达分析.植物生理学报，2018，54(01)：71-80

28.    季凌飞, 倪康, 马立锋, 陈兆杰, 赵远艳, 阮建云, 郭世伟 (2018): 不同施肥方式对酸性茶园土壤真菌群落的影响. 生态学报38(22), 8158-8166.