1.   国家自然科学基金面上项目，31972670，代谢型谷氨酸受体II组成员作为狂犬病病毒受体介导其感染的体内致病机制研究，2020/01-2023/12,直接经费58万元，主持。

2.   国家自然科学基金青年基金，81001311，甲型H1N1流感病毒与季节性流感病毒交互免疫机制的研究，2011/01-2013/12，22万元，主持。

3.   利用siRNA技术筛选狂犬病病毒复制感染相关宿主因子。SKLVBP2015003，115万元，2015.01-2017.12，兽医生物技术国家重点实验室，主持。

4.   公益性行业科研专项课题，200903037，尼帕脑炎重组病毒活载体疫苗的研究，2009/11-2013/12，125万元，骨干。

5.   “十二五”国家科技支撑计划课题，2012BAD12B05，严重威胁的外来动物疫病免疫防控储备技术，2013/01-2017/12，588万元，骨干（排名第二）。

6.   国家自然科学基金青年基金，31302064，表达禽流感病毒抗原重组鸭病毒性肠炎病毒诱导特异性快速免疫保护的机制，2014/1-2016/12，22万元，参加。

7.   国家重点研发计划项目，2020YFC0841100，2019-nCoV动物模型构建，2020/01-2020/12，1260万元，骨干。

    一、代表性论著：

1.       JianzhongShi#, Zhiyuan Wen#, Gongxun Zhong#,Huanliang Yang#, Chong Wang#, Baoying Huang#, Renqiang Liu, Xijun He, LeiShuai, Ziruo Sun, Yubo Zhao, Peipei Liu, Libin Liang, Pengfei Cui, JinliangWang, Xianfeng Zhang, Yuntao Guan, Wenjie Tan, Guizhen Wu*, Hualan Chen*,Zhigao Bu*. Susceptibility of Ferrets, Cats, Dogs, and Other DomesticatedAnimals to SARS-coronavirus 2. Science. 2020 May 29;368(6494):1016-1020.

2.       LeiShuai#, Gongxun Zhong#, Quan Yuan#, Zhiyuan Wen#, Chong Wang#, Xijun He#,Renqiang Liu, Jinliang Wang, Qinjian Zhao, Yuxiu Liu, Ningning Huo, JunhuaDeng, Jingjing Bai, Hongchao Wu, Yuntao Guan, Jianzhong Shi, Kegong Tian,Ningshao Xia, Hualan Chen, Zhigao Bu. Replication, pathogenicity, andtransmission of SARS-CoV-2 in minks. National Science Review, 8: nwaa291, 2021

3.       JinliangWang#, Lei Shuai#, Chong Wang#, Renqiang Liu#, Xijun He, Xianfeng Zhang , ZiruoSun, Dan Shan, Jinying Ge, Xijun Wang, Ronghong Hua, Gongxun Zhong*, ZhiyuanWen*, Zhigao Bu*. Mouse-adapted SARS-CoV-2 replicates efficiently in the upperand lower respiratory tract of BALB/c and C57BL/6J mice. Protein Cell. 2020Oct;11(10):776-782.

4.       JinliangWang#, Zilong Wang#, Renqiang Liu#, Lei Shuai, Xinxin Wang, Jie Luo, Chong Wang,Weiye Chen, Xijun Wang, Jinying Ge, Xijun He, Zhiyuan Wen*, Zhigao Bu*. Metabotropic glutamate receptor subtype 2is a cellular receptor for rabies virus. PLoS Pathog. 2018 Jul 20;14(7): e1007189.

5.       ChongWang#, Jinliang Wang#, Lei Shuai, Xiao Ma, Hailin Zhang, Renqiang Liu, WeiyeChen, Xijun Wang, Jinying Ge, ZhiyuanWen*, Zhigao Bu*. The Serine/Threonine kinase AP2-associated kinase 1 playsan important role in rabies virus entry. Viruses. 2019 Dec 30;12(1):45.

6.       RenqiangLiu, Jinliang Wang, Yu Shao, Xijun Wang, Huilei Zhang, Lei Shuai, Jinying Ge, Zhiyuan Wen*, Zhigao Bu*. A recombinantVSV-vectored MERS-CoV vaccine induces neutralizing antibody and T cellresponses in rhesus monkeys after single dose immunization. Antiviral Res. 2018Feb; 150:30-38.

7.       RenqiangLiu, Zhiyuan Wen*, Jinling Wang, JinyingGe, Hualan Chen, Zhigao Bu*. Absence of Middle East respiratory syndromecoronavirus in Bactrian camels in the West Inner Mongolia Autonomous Region ofChina: surveillance study results from July 2015. 2015 Emerg Microbes Infect 4:e73.

8.       DongniKong#, Zhiyuan Wen#*, Hua Su, JinyingGe, Weiye Chen, Xijun Wang, Chao Wu, Chinglai Yang, Hualan Chen, Zhigao Bu*. Newcastledisease virus-vectored Nipah encephalitis vaccines induce B and T cellresponses in mice and long-lasting neutralizing antibodies in pigs. Virology.2012 Oct 25;432(2):327-35.

9.       Zhiyuan Wen,Ling Ye, Yulong Gao, Lei Pan, Ke Dong, Zhigao Bu, Richard W Compans, Chinglai Yang*. Immunization by influenza virus-like particles protects aged mice againstlethal influenza virus challenge. Antiviral Res. 2009 Dec;84(3):215-24.

    二、论著之外的代表性研究成果和学术奖励。

期刊论文

1.       Zhiyuan Wen#,Xi Wang#, Ke Dong, Huizhong Zhang, Zhigao Bu, Ling Ye, Chinglai Yang*. Blockageof regulatory T cells augments induction of protective immune responses byinfluenza virus-like particles in aged mice. Microbes Infect. 2017Dec;19(12):626-634

2.       RenqiangLiu, Jinying Ge, Jinliang Wang, Yu Shao, Huilei Zhang, Jinling Wang, Zhiyuan Wen*, Zhigao Bu*. Newcastledisease virus-based MERS-CoV candidate vaccine elicits high-level and lastingneutralizing antibodies in Bactrian camels. Journal of Integrative Agriculture.2017 16:2264-2273.

3.       MinminZhang, Jinying Ge, Xiaofang Li, Weiye Chen, Xijun Wang, Zhiyuan Wen*, Zhigao Bu*. Protective efficacy of a recombinantNewcastle disease virus expressing glycoprotein of vesicular stomatitis virusin mice. Virol J. 2016 Feb 24;13:31.

4.       JinliangWang, Jie Yang, Jinying Ge, Ronghong Hua, Renqiang Liu, Xiaofeng Li, Xijun Wang,Yu Shao, Encheng Sun, Donglai Wu, Chengfeng Qin, Zhiyuan Wen*, Zhigao Bu*. Newcastle disease virus-vectored WestNile fever vaccine is immunogenic in mammals and poultry. Virol J. 2016 Jun 24;13:109.

5.       Zhiyuan Wen#,Bolin Zhao#, Kun Song, Xule Hu, Weiye Chen, Dongni Kong, Jinying Ge, Zhigao Bu*.Recombinant lentogenic Newcastle disease virus expressing Ebola virus GPinfects cells independently of exogenous trypsin and uses macropinocytosis asthe major pathway for cell entry. Virol J. 2013 Nov 9;10:331.

授权发明专利

1.      步志高，温志远，葛金英，华荣虹，王喜军，表达西尼罗热病毒PrM/E蛋白的重组新城疫LaSota疫苗株， 2018.04.27，中国，ZL201510036152.1

2.      步志高，温志远，葛金英，表达尼帕脑炎病毒G蛋白的重组新城疫病毒LaSota疫苗株， 2013.11.06，中国，ZL201110431883.8

3.      步志高，温志远，葛金英，表达尼帕脑炎病毒F蛋白的重组新城疫病毒LaSota疫苗株， 2013.06.12，中国，ZL201110431892.7